Over the last decade, electrolyzed water (EW) produced by salt and tap water has gained importance due to its antimicrobial effects. Regarding to chlorine-based compounds, EW also used in post-harvest safety of food processing and sterilization of surfaces. The latest studies suggested that EW might act as wound healing agent due to anti-infective and cell proliferative properties. In this study, we evaluated acute contact cytotoxicity in L929 mice fibroblast cells and wound healing activity of EWs in vitro. In addition, mutagenic activity was evaluated by Ames test with and without metabolic activation by S9 fraction and the stability profile of freshly prepared EWs has been followed up. According to the results, strong acid (StAEW) and mixed EW (MEW) showed dose-dependent cytotoxicity due to possible high HOCl concentration, while slightly acidic and catholyte EW (CEW) were not cytotoxic even applied directly for 30 sec. Further, StAEW and CEW showed a significant increase in L929 cell migration in scratch assay. Likewise, with/ without metabolic activation, neither of EWs had shown mutagenic profile in TA 98 and TA100 strains of Salmonella typhimurium. Follow-up of ORP (oxidation-reduction potential), pH and FCC (free chlorine concentration) showed that temperature and light were important storage conditions to maintain a stable profile particularly for ORP and FCC, which are the most important indicators for biological activity of EW. According to the present findings, it can be suggested that particularly StAEW, may represent a valuable wound healing agent with an achievable, economical and easy production system when stored under proper conditions.

在过去十年中，由盐水和自来水生产的电解水 (EW) 由于其抗菌作用而变得越来越重要。关于氯基化合物，电子战还用于食品加工的收获后安全和表面消毒。最新研究表明，由于抗感染和细胞增殖特性，EW 可能充当伤口愈合剂。在这项研究中，我们评估了 L929 小鼠成纤维细胞的急性接触细胞毒性和体外EW 的伤口愈合活性。此外，诱变活性通过 Ames 测试评估，有和没有 S9 部分的代谢激活，并且对新鲜制备的 EW 的稳定性进行了跟踪。根据结果​​，强酸 (StAEW) 和混合 EW (MEW) 由于可能的高 HOCl 浓度而显示出剂量依赖性细胞毒性，而弱酸性和阴极电解液 (CEW) 即使直接应用 30 秒也没有细胞毒性。此外，StAEW 和 CEW 在划痕试验中显示出 L929 细胞迁移的显着增加。同样，在有/没有代谢激活的情况下，EW 在 TA 98 和 TA100鼠伤寒沙门氏菌菌株中均未显示致突变谱。ORP（氧化还原电位）、pH 和 FCC（游离氯浓度）的跟踪表明，温度和光照是保持稳定的重要储存条件，尤其是 ORP 和 FCC，它们是生物活性的最重要指标。电子战。根据目前的研究结果，可以表明，特别是 StAEW，当储存在适当条件下时，可能代表一种有价值的伤口愈合剂，具有可实现的、经济的和容易的生产系统。