In this pathbreaking study, we evaluated nitrosative stress in morbidly obese patients with and without metabolic syndrome. 62 women with class 3 obesity () were divided into three subgroups: obese patients (OB), obese patients with hypertension (OB+HYP), and obese patients with metabolic syndrome (OB+MS). In comparison to the lean patients, OB had increased levels of serum myeloperoxidase (MPO), plasma nitric oxide (NO), S-nitrosothiols, and peroxynitrite (ONOO⁻), as well as nitrotyrosine, while oxidized glutathione (GSSG) rose only in OB+HYP group. Interestingly, ONOO⁻ was significantly higher in OB+HYP and OB+MS as compared to OB group, while MPO only in OB+MS group. OB+MS had greater nitrotyrosine and S-nitrosothiol values than OB+HYP. Moreover, peroxynitrite could differentiate OB from OB+HYP and OB+MS (AUC 0.9292; ; 87.5% sensitivity, 90% specificity) as well as between OB and OB+MS group (AUC 0.9125; ; 81.25% sensitivity, 83.33%). In conclusion, we showed that MPO activity, NO formation, and nitrosative damage to proteins parallel the progression of metabolic disturbances of obesity. Evaluation of ONOO⁻ concentrations may help predict the development of hypertension and metabolic syndrome in patients with morbid obesity; however, longer-term studies are required for larger numbers of patients.

中文翻译：

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高血压和代谢综合征对病态肥胖患者亚硝酸盐应激和谷胱甘肽代谢的影响。

在这项开创性的研究中，我们评估了患有和不患有代谢综合征的病态肥胖患者的亚硝化应激。62名3级肥胖女性（）分为三个子组：肥胖患者（OB），肥胖高血压患者（OB + HYP）和肥胖代谢综合征患者（OB + MS）。相比于瘦患者，OB增加了血清髓过氧化物酶（MPO），血浆一氧化氮（NO）的水平，S-亚硝基硫醇，和过氧亚硝酸盐（ONOO ^- ），以及硝基酪氨酸，而氧化型谷胱甘肽（GSSG）上升仅在OB + HYP组。有趣的是，ONOO ^-在OB + HYP OB + MS相比OB组作为显著更高，而仅MPO在OB + MS组。OB + MS比OB + HYP具有更高的硝基酪氨酸和S-亚硝基硫醇值。此外，过氧亚硝酸盐可以区分OB和OB + HYP和OB + MS（AUC 0.9292;; OB和OB + MS组之间的敏感性为87.5％，特异性为90％）（AUC 0.9125；; 灵敏度为81.25％，83.33％）。总之，我们表明MPO活性，NO的形成和蛋白质的亚硝化损伤与肥胖的代谢紊乱的进展平行。ONOO的评价^-浓度可能有助于预测高血压和病态肥胖患者代谢综合征的发展; 但是，大量患者需要长期研究。