We investigated the in vitro effects of Lactobacillus casei ZX633 on gut microorganism composition in infants with diarrhea. For this purpose, 103 feces samples from healthy infants (healthy group) and 300 diarrhea samples from infants (diarrhea group) were collected, and diarrhea feces were treated with L. casei ZX633, which was previously isolated from healthy infant feces (treatment group). We used microbial dilution plate methods, high performance liquid chromatography (HPLC) and high-throughput sequencing approaches to analyze viable main microorganism counts, short chain fatty acid (SCFA) concentrations, and intestinal microbiota composition in feces, respectively. Our data showed that L. casei ZX633 supplementation increased the numbers of Escherichia coli, yeasts, lactic acid bacteria (LAB) and aerobic-bacteria, raised propionic acid levels but reduced four other SCFAs, which are close to the healthy group. Alpha diversity results indicated that microbial diversity and richness decreased in treatment group. Bacterial community analyses revealed that microbial structures of the treatment group tended toward the healthy group; i.e., Escherichia-Shigella and Clostridioides abundance increased, and there was a reduction in the abundance of Streptococcus, Bacteroides, Enterococcus and Veillonella. In conclusion, L. casei ZX633 isolated from healthy infant feces, may be effective in improving infant diarrhea microbiota, potentially providing a new probiotic strain to reduce the incidence of diarrhea associated with bacterial disease in infants.

我们调查了 体外 的影响 干酪乳杆菌ZX633对腹泻婴儿肠道微生物组成的影响。为此，收集了103例健康婴儿（健康组）的粪便样本和300例婴儿（腹泻组）的腹泻样本，并对腹泻​​粪便进行了处理。干酪乳杆菌ZX633，先前从健康的婴儿粪便中分离出来（治疗组）。我们使用微生物稀释板法，高效液相色谱（HPLC）和高通量测序方法分别分析了粪便中可行的主要微生物数量，短链脂肪酸（SCFA）浓度和肠道菌群组成。我们的数据表明干酪乳杆菌 大肠杆菌 大肠埃希氏菌 和 梭菌 链球菌，拟杆菌，肠球菌 和 Veillonella 干酪乳杆菌