This study investigated the optimal concentration of hydrogen peroxide (H2O2) for demonstrating glycogen in rabbit liver using Schiff's reaction as compared to the conventional periodic acid-Schiff (PAS) method. Four fixatives, Bouin's, neutral buffered formalin (NBF), 80% ethanol, and Rossman's, were used at room temperature (RT) and 4°C. Paraffin sections of rabbit liver (n = 192) were stained using 0.5%, 1% and 2% periodic acid-Schiff (PAS) and 0.5%, 1%, and 2% hydrogen peroxide (H2O2)-Schiff methods. It was found that 0.5% H2O2-Schiff provided good staining results, while 1% PAS produced excellent results for glycogen fixed with 80% ethanol at RT. Also, 0.5% H2O2-Schiff produced good staining of glycogen fixed with 10% NBF at RT as compared to 0.5% PAS which provided satisfactory staining results. Fixation with 80% ethanol at RT produced the best staining quality for glycogen using both oxidizing agents at the optimal concentrations of either 1% periodic acid or 0.5% H2O2.

中文翻译：

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过氧化氢作为氧化剂在用酒精和水性固定剂固定的兔肝脏中使用希夫试剂进行糖原证明

与传统的高碘酸-希夫 (PAS) 方法相比，本研究调查了使用希夫反应证明兔肝脏中糖原的最佳过氧化氢 (H2O2) 浓度。四种固定剂，Bouin's、中性缓冲福尔马林 (NBF)、80% 乙醇和 Rossman's，在室温 (RT) 和 4°C 下使用。兔肝脏 (n = 192) 的石蜡切片使用 0.5%、1% 和 2% 高碘酸-希夫 (PAS) 和 0.5%、1% 和 2% 过氧化氢 (H2O2)-希夫方法染色。发现 0.5% H2O2-Schiff 提供了良好的染色结果，而 1% PAS 对用 80% 乙醇在室温下固定的糖原产生了极好的结果。此外，与提供令人满意的染色结果的 0.5% PAS 相比，0.5% H2O2-Schiff 对用 10% NBF 固定的糖原产生了良好的染色。