Abstract

In recent years, there has been increasing interest in microbial biotechnology for the production of value-added compounds from renewable resources. Pseudomonas species have been proposed as a suitable workhorse for high-value secondary metabolite production because of their unique characteristics for fast growth on sustainable carbon sources, a clear inherited background, versatile intrinsic metabolism with diverse enzymatic capacities, and their robustness in an extreme environment. It has also been demonstrated that metabolically engineered Pseudomonas strains can produce several industrially valuable aromatic chemicals and natural products such as phenazines, polyhydroxyalkanoates, rhamnolipids, and insecticidal proteins from renewable feedstocks with remarkably high yields suitable for commercial application. In this review, we summarize cell factory construction in Pseudomonas for the biosynthesis of native and non-native bioactive compounds in P. putida, P. chlororaphis, P. aeruginosa, as well as pharmaceutical proteins production by P. fluorescens. Additionally, some novel strategies together with metabolic engineering strategies in order to improve the biosynthetic abilities of Pseudomonas as an ideal chassis are discussed. Finally, we proposed emerging opportunities, challenges, and essential strategies to enable the successful development of Pseudomonas as versatile microbial cell factories for the bioproduction of diverse bioactive compounds.

摘要

近年来，人们对利用可再生资源生产增值化合物的微生物生物技术越来越感兴趣。假单胞菌属物种已被提出作为高价值次级代谢产物生产的合适主力军，因为它们具有在可持续碳源上快速生长的独特特征，清晰的遗传背景，具有多种酶解能力的内在新陈代谢以及在极端环境下的坚固性。也已经证明，代谢工程假单胞菌该菌株可以从可再生原料中生产出几种工业上有价值的芳香族化学品和天然产物，例如吩嗪，聚羟基链烷酸酯，鼠李糖脂和杀虫蛋白，其高收率非常适合商业应用。在这篇综述中，我们总结了假单胞菌中用于天然合成P和非天然生物活性化合物的细胞工厂。恶臭，P。chlororaphis，P。铜绿假单胞菌以及P的药用蛋白生产。荧光素。此外，讨论了一些新策略以及代谢工程策略，以提高假单胞菌作为理想底盘的生物合成能力。最后，我们提出了新的机遇，挑战和基本策略，以使假单胞菌成功发展为多功能微生物细胞工厂，用于生物生产各种生物活性化合物。