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Investigating Byssogenesis with Proteomic Analysis of Byssus, Foot and Mantle in Mytilus Mussels by LC-MS/MS.
Proteomics ( IF 3.4 ) Pub Date : 2020-09-10 , DOI: 10.1002/pmic.202000014
Maxime Sansoucy 1 , Réjean Tremblay 2 , Emily Carrington 3 , Isabelle Marcotte 1 , Lekha Sleno 1
Affiliation  

Mussel byssus represents a fascinating class of biological materials with a unique capacity to adhere onto virtually any solid surface. Proteins expressed in byssus, the byssal‐producing organ (foot) as well as mantle tissue from Mytilus edulis or Mytilus californianus are analyzed by liquid chromatography tandem mass spectrometry (LC‐MS/MS). The mantle is used as a control tissue to pinpoint unique proteins from the foot samples potentially involved in byssogenesis. This work represents an important step towards identifying biologically important proteins expressed in foot, as well as extending knowledge on the byssus proteome. Considering the minimal proteomics data of the studied species, this data also contributes to a more complete description of M. edulis and M. californianus proteomes.

中文翻译:

通过 LC-MS/MS 对贻贝中的 Byssus、足部和地幔进行蛋白质组学分析,研究 Byssogenesis。

Mussel byssus 代表了一类迷人的生物材料,具有粘附在几乎任何固体表面上的独特能力。通过液相色谱串联质谱法 (LC-MS/MS) 分析了在贝壳、产生贝壳的器官(足)以及来自Mytilus edulisMytilus californianus 的地幔组织中表达的蛋白质。地幔被用作对照组织,以从可能参与合成的足部样本中找出独特的蛋白质。这项工作代表了鉴定足部表达的生物学上重要的蛋白质以及扩展对 byssus 蛋白质组知识的重要一步。考虑到所研究物种的最少蛋白质组学数据,该数据也有助于更完整地描述M. edulisM. californianus蛋白质组。
更新日期:2020-09-10
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