High expression of suppressors of cytokine signalling (SOCS) has been detected during various viral infections. As a negative feedback regulator, SOCS participates in the regulation of multiple signalling pathways. In this study, to study the related mechanism between SOCS and BDV and to explore the effect of SOCS on IFN pathways in nerve cells, downregulated of SOCS1/3 in oligodendroglial (OL) cells and OL cells persistently infected with BDV (OL/BDV) were constructed with RNA interference technology. An interferon inducer (poly I:C, PIC) and an IFN‐α/β R1 antibody were used as stimulation in the SOCS1/3 low‐expression cell models, qRT‐PCR was used to detect type I IFN and BDV nucleic acid expression, Western blot was used to detect the expression of BDV P40 protein. After BDV acute infection with OL cells which with downregulated SOCS expression, the virus accounting was not detected, and the viral protein expression was lower than that of OL/BDV cells; the OL/BDV cells with downregulated SOCS expression had lower virus nucleic acid and protein expression than OL/BDV cells. Stimulated by IFN‐α/β R1 antibody, the expression of type I interferon in OL/BDV cells decreased, and the content of BDV nucleic acid and protein increased, which was higher than that of OL/BDV cells. From the results, it was concluded that downregulating SOCS1/3 can inhibit the formation of acute BDV infection and virus replication in persistent BDV infection by promoting the expression of IFN‐α/β and that SOCS can be used as a new target for antiviral therapy.

中文翻译：

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SOCS基因表达的下调可以抑制急性和持续性BDV感染的形成。

在各种病毒感染期间已检测到细胞因子信号转导（SOCS）抑制剂的高表达。作为负反馈调节剂，SOCS参与多种信号通路的调节。在这项研究中，研究SOCS与BDV之间的相关机制，并探讨SOCS对神经细胞IFN途径的影响，少突胶质（OL）细胞和持续感染BDV（OL / BDV）的OL细胞中SOCS1 / 3的下调。用RNA干扰技术构建。在SOCS1 / 3低表达细胞模型中，干扰素诱导剂（poly I：C，PIC）和IFN-α/βR1抗体被用作刺激，qRT-PCR被用于检测I型干扰素和BDV核酸表达用Western blot检测BDV P40蛋白的表达。BDV急性感染了SOCS表达下调的OL细胞后，未检测到病毒，病毒蛋白表达低于OL / BDV细胞。SOCS表达下调的OL / BDV细胞的病毒核酸和蛋白质表达低于OL / BDV细胞。在IFN-α/βR1抗体的刺激下，OL / BDV细胞中I型干扰素的表达下降，BDV核酸和蛋白质的含量增加，高于OL / BDV细胞。从结果可以得出结论，下调SOCS1 / 3可以通过促进IFN-α/β的表达抑制持续BDV感染中急性BDV感染的形成和病毒复制，并且SOCS可以用作抗病毒治疗的新靶点。SOCS表达下调的OL / BDV细胞的病毒核酸和蛋白质表达低于OL / BDV细胞。在IFN-α/βR1抗体的刺激下，OL / BDV细胞中I型干扰素的表达下降，BDV核酸和蛋白质的含量增加，高于OL / BDV细胞。从结果可以得出结论，下调SOCS1 / 3可以通过促进IFN-α/β的表达抑制持续BDV感染中急性BDV感染的形成和病毒复制，并且SOCS可以用作抗病毒治疗的新靶点。SOCS表达下调的OL / BDV细胞的病毒核酸和蛋白质表达低于OL / BDV细胞。在IFN-α/βR1抗体的刺激下，OL / BDV细胞中I型干扰素的表达下降，BDV核酸和蛋白质的含量增加，高于OL / BDV细胞。从结果可以得出结论，下调SOCS1 / 3可以通过促进IFN-α/β的表达抑制持续BDV感染中急性BDV感染的形成和病毒复制，并且SOCS可以用作抗病毒治疗的新靶点。高于OL / BDV细胞。从结果可以得出结论，下调SOCS1 / 3可以通过促进IFN-α/β的表达抑制持续BDV感染中急性BDV感染的形成和病毒复制，并且SOCS可以用作抗病毒治疗的新靶点。高于OL / BDV细胞。从结果可以得出结论，下调SOCS1 / 3可以通过促进IFN-α/β的表达抑制持续BDV感染中急性BDV感染的形成和病毒复制，并且SOCS可以用作抗病毒治疗的新靶点。