Circular RNA (circRNA) is a novel noncoding RNA that is mostly found in humans and animals. Although the flux of circRNA research has increased in recent years, its precise function is still unclear. Some studies demonstrate that circRNAs can function as microRNA (miRNA) sponges involved in the regulation of competitive endogenous RNAs networks and play a crucial role in many biological processes. Other studies show that circRNAs play multiple biological roles in gastrointestinal diseases. However, the expression characteristics and function of circRNA in intestinal mucosal injury and repair after severe burn have not been reported. This study aims to screen differentially expressed circRNAs in intestinal mucosal injury and repair after severe burns and understand their underlying mechanisms. To test our hypothesis that circRNA may play a role in promoting repair in intestinal mucosa injury after severe burns, we collected the intestinal tissues of three severely burned mice and three pseudo‐scalded mice and evaluated the expression of circRNAs via microarray analysis. Quantitative real‐time polymerase chain reaction was also used to validate the circRNA microarray data by selecting six based on different multiples, original values, and p values. The host genes of all differentially expressed circRNAs and the downstream target genes of six selected DEcircRNAs were identified by Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Meanwhile, we also created a circRNA‐miRNA‐mRNA network to predict the role and function of circRNAs in intestinal mucosal injury and repair after severe burns.

中文翻译：

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严重烧伤后肠黏膜损伤和修复中环状RNA的表达谱和生物信息学分析。

环状RNA（circRNA）是一种新型的非编码RNA，主要存在于人和动物中。尽管近年来circRNA研究的流量不断增加，但其确切功能仍不清楚。一些研究表明，circRNA可以充当参与竞争性内源RNA网络调控的microRNA（miRNA）海绵，并在许多生物学过程中发挥关键作用。其他研究表明，circRNA在胃肠道疾病中起多种生物学作用。然而，尚未报道circRNA在严重烧伤后肠粘膜损伤和修复中的表达特征和功能。这项研究的目的是筛选严重烧伤后肠黏膜损伤和修复中差异表达的circRNA，并了解其潜在机制。为了检验circRNA可能在严重烧伤后促进肠道粘膜损伤修复中发挥作用的假设，我们收集了三只严重烧伤小鼠和三只假烫伤小鼠的肠道组织，并通过微阵列分析评估了circRNA的表达。实时定量聚合酶链反应还用于通过基于不同的倍数，原始值和p 值。通过基因本体论和《京都议定书》全书的基因和基因组途径分析，鉴定了所有差异表达的circRNA的宿主基因和六个选定的DEcircRNA的下游靶基因。同时，我们还创建了circRNA-miRNA-mRNA网络，以预测circRNA在严重烧伤后肠道粘膜损伤和修复中的作用和功能。