In this study, the recovery of different bioactive compounds from Nannochloropsis oceanica biomass is proposed using high and ultra-high pressure-based processes. The effect of different pretreatments to weaken cell wall was evaluated before pressurized liquid extraction (PLE). PLE was optimized using an experimental design with ethanol as extraction solvent. Optimum extraction conditions were found at 57 °C and 3 extraction cycles, providing a total carotenoids content of 115.1 ± 0.6 mg/g extract. Then, the residual biomass was extracted using limonene, and an enriched-lipid fraction was obtained. In parallel, ultra-high pressure extraction (UHPE) was applied directly to dry biomass to simultaneously disrupt the cell wall and extract the valuable compounds. Results showed that UHPE technique did not improve carotenoids extraction compared to PLE, although the recovery of polyunsaturated fatty acids was significantly better. Moreover, extracts were chemically characterized using high-performance liquid chromatography detection and gas chromatography coupled to mass spectrometry detection.

在这项研究中，从楠拟南芥中回收不同生物活性化合物提出使用基于高压和超高压的工艺来生产生物质。在加压液体提取（PLE）之前评估了不同预处理对削弱细胞壁的影响。使用乙醇作为萃取溶剂的实验设计对PLE进行了优化。最佳的提取条件是在57°C和3个提取循环中发现的，总类胡萝卜素含量为115.1±0.6 mg / g提取物。然后，使用柠檬烯提取残留的生物质，并获得富集脂质的馏分。同时，将超高压提取（UHPE）直接应用于干燥的生物质，以同时破坏细胞壁并提取有价值的化合物。结果表明，尽管多不饱和脂肪酸的回收率要好得多，但与PLE相比，UHPE技术并不能改善类胡萝卜素的提取。此外，