Abstract Numerous studies investigated the biosynthesis of silver nanoparticles (AgNPs); however, there is a large gap for the ideal time-consuming process and their cytotoxicity. Herein, for the first time, rapid AgNPs was synthesized in a short time span, using Piper betle leaf (PBL) extract by applying microwave exposure. PB-AgNPs antibacterial activity and cell compatibility were enhanced by capping with chitosan (CS@PB-AgNPs). The synthesized nanoparticles were characterized by bioanalytical techniques. PB-AgNPs expressed significant antibacterial activity against Gram-positive and Gram-negative bacterial pathogens, while hybrid CS@PB-AgNPs presented the enhanced bactericidal activity. In addition, PB-AgNPs exhibited IC50 value of 140 μg/mL against RAW 264.7 macrophages and 100 μg/mL against lung cancer cells while, CS capping reduced its toxicity at IC50 values of 400 μg/mL and 180 μg/mL respectively were affirmed by MTT, apoptosis and DNA damage detection. Overall it was demonstrated that CS capping could be a phenomenal finding to improve the biomedical potential of AgNPs.

中文翻译：

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生物相容性壳聚糖修饰银纳米粒子生物复合材料的合成以增强抗菌和抗癌性能

摘要 大量研究调查了银纳米粒子 (AgNPs) 的生物合成；然而，理想的耗时过程及其细胞毒性存在很大差距。在此，首次使用蒺藜叶 (PBL) 提取物通过微波照射在短时间内合成了快速 AgNPs。PB-AgNPs 的抗菌活性和细胞相容性通过壳聚糖（CS@PB-AgNPs）封端增强。合成的纳米颗粒通过生物分析技术进行表征。PB-AgNPs 对革兰氏阳性和革兰氏阴性细菌病原体表现出显着的抗菌活性，而混合 CS@PB-AgNPs 则表现出增强的杀菌活性。此外，PB-AgNPs 对 RAW 264.7 巨噬细胞的 IC50 值为 140 μg/mL，对肺癌细胞的 IC50 值为 100 μg/mL，通过 MTT、细胞凋亡和 DNA 损伤检测证实，CS 封端在 IC50 值分别为 400 μg/mL 和 180 μg/mL 时降低了其毒性。总体而言，已证明 CS 封端可能是提高 AgNPs 生物医学潜力的惊人发现。