Abstract Biofilm-associated Candida albicans infection remains to be challenging, though there are several investigations and developments in the medical therapy. Antimicrobial peptides are promising candidates due to their efficacy to target pathogens and cancer cells. In the present study, LLpepII identified from Linckia laevigata (star fish), has been predicted as ɑ-helical, amphipathic antimicrobial peptide. LLpepII was investigated for their potency to inhibit C. albicans biofilm formation using crystal violet. LLpepII shows antibiofilm activity which inhibits only the biofilm formation and without inhibiting the growth of the yeast cells. This biofilm specific activity of LLpepII was confirmed through SEM and XTT assay. Cell viability assay revealed that LLpepII significantly inhibited the proliferation of human cervical (HeLa) cell lines. Morphological changes in HeLa cells, apoptosis and cell death was studied using AO/EB. It is also confirmed that LLpepII can prevent infection and it is non toxic using zebrafish egg infection model. These results suggest that amphipathic LLpepII as promising candidate with dual property can be explored further as antibiofilm and antiproliferate candidate for therapeutic applications.

中文翻译：

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来自 Linckia laevigata 的抗微生物肽 (AMP) 对白色念珠菌的潜在抗生物膜特性的研究：一项体外和体内研究

摘要生物膜相关的白色念珠菌感染仍然具有挑战性，尽管在药物治疗方面有一些研究和发展。抗菌肽是有希望的候选者，因为它们具有靶向病原体和癌细胞的功效。在本研究中，从 Linckia laevigata（海星）中鉴定出的 LLpepII 被预测为 ɑ-螺旋、两亲性抗菌肽。使用结晶紫研究了 LLpepII 抑制白色念珠菌生物膜形成的效力。LLpepII 显示出抗生物膜活性，该活性仅抑制生物膜形成而不抑制酵母细胞的生长。LLpepII 的这种生物膜比活性通过 SEM 和 XTT 测定得到证实。细胞活力测定显示 LLpepII 显着抑制人宫颈 (HeLa) 细胞系的增殖。使用 AO/EB 研究 HeLa 细胞的形态学变化、细胞凋亡和细胞死亡。LLpepII 也证实了 LLpepII 可以预防感染并且使用斑马鱼卵感染模型无毒。这些结果表明，两亲性 LLpepII 作为具有双重特性的有前途的候选物可以进一步探索作为治疗应用的抗生物膜和抗增殖候选物。