Abstract Copper complexes were synthesized via thiol complexation with either d or l -penicillamine (DP and LP), l -cysteine (LC) and reduced glutathione (GR) by solution-precipitation method. Complex formation was demonstrated by thiol disappearance by FTIR and Raman. DP, LC and GR complexes were crystalline as shown by XRD, but LC and GR complexes exhibited higher decomposition temperatures as shown by TGA. DP and LP copper complexes were cytotoxic to macrophages in the 800 to 1600 µM range but not for LC and only at 1600 µM for GR complexes. The later complexes slightly suppressed IL-1β, IL-6 and TNF-α production while enhancing IL-4 and IL-10 expression. These results suggest that LC and GR based copper complexes can be promising fillers or coatings for biomedical applications.

中文翻译：

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异位硫醇基铜螯合物的非炎症特性

摘要 铜配合物是通过硫醇与 d 或 l-青霉胺（DP 和 LP）、l-半胱氨酸 (LC) 和还原型谷胱甘肽 (GR) 通过溶液沉淀法合成的。FTIR 和拉曼通过硫醇消失证明了复合物的形成。DP、LC 和 GR 配合物如 XRD 所示是结晶的，但如 TGA 所示，LC 和 GR 配合物表现出更高的分解温度。DP 和 LP 铜复合物在 800 到 1600 µM 范围内对巨噬细胞具有细胞毒性，但对 LC 没有，而仅在 1600 µM 时对 GR 复合物具有细胞毒性。后来的复合物略微抑制了 IL-1β、IL-6 和 TNF-α 的产生，同时增强了 IL-4 和 IL-10 的表达。这些结果表明，基于 LC 和 GR 的铜配合物可以作为生物医学应用的有前途的填料或涂层。