Abstract Anaerobic ammonium oxidation (anammox) is an energy saving and environmentally friendly technique for wastewater treatment. Sludge adsorption is an important process after organics enter the anammox reactor. The extracellular polymeric substances (EPS) of anammox sludge play a key role on the activity of anammox. This study utilized Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to investigate molecular-level compositional characteristics of the stratified EPS of the anammox sludge, and to explore the adsorption preference of each EPS layer to refractory dissolved organic matter (DOM) during anammox treatment. Results showed that the adsorbed component by the tightly bound EPS layer was dominantly composed of lipids and proteins, with nearly 80% of formulas being CHO and CHON. The outer layers (slime, loosely bound-EPS) preferentially adsorbed the compounds with comparatively higher aromatic and unsaturated degrees, with S-containing formulas and lignin being the predominant components. The newly produced formulas in the effluent were inferred to be associated with the anammox treatment performance. The refractory DOM, with smaller molecular weight and high reductive degree, seemed to experience a single adsorption by the stratified EPS, and became part of the anammox sludge.

中文翻译：

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厌氧氨氧化污泥分层胞外聚合物的分子水平表征及其对难降解溶解有机物的吸附偏好

摘要 厌氧氨氧化（anammox）是一种节能环保的废水处理技术。污泥吸附是有机物进入厌氧氨氧化反应器后的一个重要过程。厌氧氨氧化污泥的胞外聚合物（EPS）对厌氧氨氧化的活性起着关键作用。本研究利用傅里叶变换离子回旋共振质谱（FT-ICR-MS）研究了厌氧氨氧化污泥分层EPS的分子水平组成特征，并探讨了每个EPS层对难降解溶解有机物的吸附偏好。 DOM) 在厌氧氨氧化处理期间。结果表明，紧密结合的EPS层吸附的成分主要由脂质和蛋白质组成，近80%的配方为CHO和CHON。外层（粘液，松散结合的EPS）优先吸附芳香度和不饱和度相对较高的化合物，其中含硫分子式和木质素是主要成分。推测出水中新产生的配方与厌氧氨氧化处理性能有关。难降解的 DOM 分子量较小，还原度较高，似乎经历了分层 EPS 的单一吸附，成为厌氧氨氧化污泥的一部分。