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The epoxy-tannic-modified crosslinked chitosan matrix with a selectively adsorptive activity towards charged proteins
Colloid and Interface Science Communications ( IF 4.7 ) Pub Date : 2020-09-10 , DOI: 10.1016/j.colcom.2020.100317
Li-Juan Chai , Yang Deng , Yinglian Zhu , Meizi Piao , Wei Xu , Yan Li , Jinwei Wang , Jian Li

Chitosan-based matrices have been proposed for the design of absorbents with target-selective activity, because they are easily modifiable, are cationic polyelectrolytes, and can be regenerated. In this study, chitosan monomers were folded into special three-dimensional structures by crosslinking them with epichlorohydrin and tannic acid. The introduction of active and charged groups such as epoxy and phenolic hydroxyl and synergistic effects among these groups produce chitosan matrices with differing abilities to adsorb proteins such as bovine serum albumin, ovalbumin, myoglobin, and lysozyme in an acidic environment. The adsorptive efficiency of proteins was evaluated via liquid chromatography. Bovine serum albumin and myoglobin were progressively adsorbed while ovalbumin was bound due to strong affinity and lysozyme was saturated in the initial stage. The protein selectivity was primarily dependent on molecular weight, molecular size, isoelectric point, and coulombic attraction. The study illustrated varying effectiveness of charged protein adsorption as well as different adsorption profiles.



中文翻译:

环氧鞣酸改性的交联壳聚糖基质,对带电蛋白质具有选择性吸附活性

已经提出了基于壳聚糖的基质用于设计具有目标选择性活性的吸收剂,因为它们易于改性,为阳离子聚电解质并且可以再生。在这项研究中,壳聚糖单体通过与环氧氯丙烷和单宁酸交联而折叠成特殊的三维结构。活性基团和带电基团(例如环氧基和酚羟基)的引入以及这些基团之间的协同作用产生了在酸性环境中具有不同能力的壳聚糖基质吸附蛋白质,如牛血清白蛋白,卵清蛋白,肌红蛋白和溶菌酶。通过液相色谱评估蛋白质的吸附效率。牛血清白蛋白和肌红蛋白被逐步吸附,而卵白蛋白由于强亲和力而被结合,溶菌酶在初始阶段就饱和。蛋白质的选择性主要取决于分子量,分子大小,等电点和库仑引力。该研究说明了带电蛋白质吸附的有效性以及不同的吸附曲线。

更新日期:2020-09-10
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