Two undescribed polyoxygenated furanocembranoid derivatives, methyl 15-(9-hydroxy-8-methoxy-2,12-dimethyl-15-oxa-bicyclo[10.2.1]pentadeca-2,4,6,11-tetraen-3-yl)propanoate (salmacembrane A) and 1-(16-methyl-2,16-dihydrofuran)-8-methoxy-12-methyl-20-oxabicyclo[10.2.1]pentadeca-2,4,6,11-tetraen-9-ol (salmacembrane B), were isolated from the organic extract of sea urchin Salmacis bicolor (family Temnopleuridae) by extensive chromatographic purification. Their structures were elucidated using detailed spectroscopic evidence. Salmacembrane A displayed significantly greater attenuation property against pro-inflammatory cyclooxygenase-2 (IC₅₀ 1.71 mM) than that exhibited by salmacembrane B (IC₅₀ 1.99 mM). Salmacembrane A could potentially inhibit 5-lipoxygenase (IC₅₀ 1.87 mM) and its activity was significantly greater than that exhibited by anti-inflammatory agent ibuprofen (IC₅₀ 4.50 mM, p < 0.05). The greater selectivity index (anti-cyclooxygense-2/anti-cyclooxygense-1) of salmacembrane A (1.06) than ibuprofen (0.43) further supported higher selectivity toward pro-inflammatory isoenzyme cyclooxygenase-2. Salmacembrane A displayed higher antioxidant properties against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl (IC₅₀ 1.57 and 1.65 mM, respectively) than those exhibited by salmacembrane B (IC₅₀ > 1.85 mM). In addition, these antioxidant activities were comparable to the standard α-tocopherol (IC₅₀ DPPH 1.51 mM, IC₅₀ ABTS⁺ 1.70 mM p < 0.05). The higher electronic parameters obtained from structure–activity relationship analysis along with greater binding affinities of salmacembrane A at the active site of cyclooxygenase-2 ascribed its potential anti-inflammatory activity.

两种未描述的多氧呋喃呋喃类衍生物，甲基15-（9-羟基-8-甲氧基-2,12-二甲基-15-氧杂双环[10.2.1] pentadeca-2,4,6,11-四烯-3-基）丙酸酯（盐藻膜A）和1-（16-甲基-2,16-二氢呋喃）-8-甲氧基-12-甲基-20-氧杂双环[10.2.1] pentadeca-2,4,6,11-tetraen-9-通过广泛的色谱纯化从海胆Salmacis bicolor（Temnopleuridae家族）的有机提取物中分离出醇（Salmacembrane B）。使用详细的光谱学证据阐明了它们的结构。相较于Salmacembrane B（IC ₅₀ 1.99 mM），Salmacembrane A对促炎性环氧合酶2（IC ₅₀ 1.71 mM）表现出更大的衰减特性。Salmacembrane A可能会抑制5-脂氧合酶（IC_50. 1.87 mM），其活性显着高于抗炎药布洛芬（IC ₅₀ 4.50 mM，p  <0.05）。相比于布洛芬（0.43），柳树膜A（1.06）的选择性指数（anti-cyclooxygense-2 / anti-cyclooxygense-1）更高，进一步支持了对促炎同工酶环氧合酶2的更高选择性。相比于金银膜，Salmacembrane A对2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）和2,2-二苯基-1-吡啶并肼基（IC ₅₀分别为1.57和1.65 mM）表现出更高的抗氧化性能。B（IC ₅₀  > 1.85 mM）。此外，这些抗氧化活性与标准α-生育酚（IC ₅₀DPPH 1.51 mM，IC ₅₀ ABTS ⁺ 1.70 mM p  <0.05）。从结构-活性关系分析获得的更高的电子参数，以及在加氧合酶-2的活性位点上具有更大的结合度的salmacembrane A归因于其潜在的抗炎活性。