We studied the expression of activation markers CD25 and CD69 by blood lymphocytes in white mice vaccinated with Brucella abortus 19 BA in antigen-specific tests in vitro. During incubation of blood lymphocytes with brucellosis polysaccharide-protein antigen, a statistically significant increase in the expression of CD25 by B cells and CD69 by T cells was observed; brucellin increased the expression of CD25 by B and T cells. Comparative analysis of the action of antigen preparations B. abortus showed that only brucellin has antigen-specific activity against CD19+CD25+ cells. The used method can be considered as a promising test for evaluation of the effectiveness of brucellosis immunoprophylaxis, which substantiates the need for further research.

中文翻译：

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流产布鲁氏菌 19 BA 免疫小鼠血淋巴细胞的抗原特异性激活

我们在体外抗原特异性测试中研究了接种流产布鲁氏菌 19 BA 的白鼠中血液淋巴细胞激活标记 CD25 和 CD69 的表达。在血液淋巴细胞与布鲁氏菌病多糖蛋白抗原孵育期间，观察到 B 细胞 CD25 和 T 细胞 CD69 表达的统计学显着增加；Brucellin 增加 B 细胞和 T 细胞的 CD25 表达。抗原制剂作用的比较分析表明，只有布鲁氏菌素对 CD19+CD25+ 细胞具有抗原特异性活性。所使用的方法可以被认为是评估布鲁氏菌病免疫预防有效性的有希望的测试，这证实了进一步研究的必要性。