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Morin Hydrate Inhibits Influenza Virus entry into Host Cells and Has Anti-inflammatory Effect in Influenza-infected Mice
Immune Network ( IF 4.3 ) Pub Date : 2020-01-01 , DOI: 10.4110/in.2020.20.e32
Eun-Hye Hong 1 , Jae-Hyoung Song 1, 2 , Seong-Ryeol Kim 1 , Jaewon Cho 1 , Birang Jeong 3 , Heejung Yang 3 , Jae-Hyeon Jeong 1 , Jae-Hee Ahn 1 , Hyunjin Jeong 1 , Seong-Eun Kim 1 , Sun-Young Chang 4 , Hyun-Jeong Ko 1, 2
Affiliation  

Influenza virus is the major cause of seasonal and pandemic flu. Currently, oseltamivir, a potent and selective inhibitor of neuraminidase of influenza A and B viruses, is the drug of choice for treating patients with influenza virus infection. However, recent emergence of oseltamivir-resistant influenza viruses has limited its efficacy. Morin hydrate (3,5,7,2′,4′-pentahydroxyflavone) is a flavonoid isolated from Morus alba L. It has antioxidant, anti-inflammatory, neuroprotective, and anticancer effects partly by the inhibition of the NF-кB signaling pathway. However, its effects on influenza virus have not been studied. We evaluated the antiviral activity of morin hydrate against influenza A/Puerto Rico/8/1934 (A/PR/8; H1N1) and oseltamivir-resistant A/PR/8 influenza viruses in vitro. To determine its mode of action, we carried out time course experiments, and time of addition, hemolysis inhibition, and hemagglutination assays. The effects of the co-administration of morin hydrate and oseltamivir were assessed using the murine model of A/PR/8 infection. We found that morin hydrate reduced hemagglutination by A/PR/8 in vitro. It alleviated the symptoms of A/PR/8-infection, and reduced the levels of pro-inflammatory cytokines and chemokines, such as TNF-α and CCL2, in infected mice. Co-administration of morin hydrate and oseltamivir phosphate reduced the virus titers and attenuated pulmonary inflammation. Our results suggest that morin hydrate exhibits antiviral activity by inhibiting the entry of the virus.

中文翻译:

Morin Hydrate 抑制流感病毒进入宿主细胞并对流感感染小鼠具有抗炎作用

流感病毒是季节性和大流行性流感的主要原因。目前,奥司他韦是甲型和乙型流感病毒神经氨酸酶的强效选择性抑制剂,是治疗流感病毒感染患者的首选药物。然而,最近出现的耐奥司他韦流感病毒限制了其疗效。Morin hydrate (3,5,7,2',4'-pentahydroxyflavone) 是一种从桑树中分离的黄酮类化合物。它具有抗氧化、抗炎、神经保护和抗癌作用,部分是通过抑制 NF-кB 信号通路. 然而,尚未研究其对流感病毒的影响。我们在体外评估了莫林水合物对甲型流感/波多黎各/8/1934(A/PR/8;H1N1)和耐奥司他韦的 A/PR/8 流感病毒的抗病毒活性。为了确定其作用方式,我们进行了时间进程实验、添加时间、溶血抑制和血凝试验。使用 A/PR/8 感染的鼠模型评估了桑椹水和奥司他韦共同给药的效果。我们发现,morin 水合物在体外降低了 A/PR/8 的血凝作用。它减轻了 A/PR/8 感染的症状,并降低了受感染小鼠中促炎细胞因子和趋化因子(如 TNF-α 和 CCL2)的水平。Morin 水合物和奥司他韦磷酸盐的共同给药降低了病毒滴度并减轻了肺部炎症。我们的结果表明,morin hydrate 通过抑制病毒进入而表现出抗病毒活性。使用 A/PR/8 感染的鼠模型评估了桑椹水和奥司他韦共同给药的效果。我们发现,morin 水合物在体外降低了 A/PR/8 的血凝作用。它减轻了 A/PR/8 感染的症状,并降低了受感染小鼠中促炎细胞因子和趋化因子(如 TNF-α 和 CCL2)的水平。Morin 水合物和奥司他韦磷酸盐的共同给药降低了病毒滴度并减轻了肺部炎症。我们的结果表明,morin hydrate 通过抑制病毒进入而表现出抗病毒活性。使用 A/PR/8 感染的鼠模型评估了桑椹水和奥司他韦共同给药的效果。我们发现,morin 水合物在体外降低了 A/PR/8 的血凝作用。它减轻了 A/PR/8 感染的症状,并降低了受感染小鼠中促炎细胞因子和趋化因子(如 TNF-α 和 CCL2)的水平。Morin 水合物和奥司他韦磷酸盐的共同给药降低了病毒滴度并减轻了肺部炎症。我们的结果表明,morin hydrate 通过抑制病毒进入而表现出抗病毒活性。在受感染的小鼠中。Morin 水合物和奥司他韦磷酸盐的共同给药降低了病毒滴度并减轻了肺部炎症。我们的结果表明,morin hydrate 通过抑制病毒进入而表现出抗病毒活性。在受感染的小鼠中。Morin 水合物和奥司他韦磷酸盐的共同给药降低了病毒滴度并减轻了肺部炎症。我们的结果表明,morin hydrate 通过抑制病毒进入而表现出抗病毒活性。
更新日期:2020-01-01
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