In the case of Tenebrionidae family insects, studies focus on larval stage, leaving a lack of information regarding other stages. Therefore, this study was performed in order to understand the differences between the nutritional composition and the bioactivity of two species of this family in their adult stage, fed with a specific diet. Adult beetles of both species were defatted, lyophilized and protein extracted with buffer. Proximal and phytochemical analysis of the extracts of each insect were performed, along with protein extract and hydrolysis analysis by Tris-Tricine and Tris Glycine SDS PAGE. This analysis showed that T. molitor contained more protein and fat than U. dermestoides but contained less crude fiber. The protein extraction was made with PBS, where 130 and 45 kDa bands showed predominant for U. dermestoides, and less protein was present for T. molitor. Antioxidant and antimicrobial activities of the enzymatic protein hydrolysates and protein crude extracts were determined. Presence of protein associated with the antioxidant activity were found in both insects. Nonetheless U. dermestoides had a higher antioxidant activity with the protein extract in contrast with the higher antioxidant activity shown by U. dermestoides once the extracts were digested. After proteolysis, protein extracts showed an increasing antioxidant activity, plus, the ability to inhibit microbial growth of Proteus, Shigella and Bacillus. Insect protein hydrolysates with protease open the possibility for the use of these beetles as new sources of encrypted peptides for microbiological control once characterized.

中文翻译：

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两种拟步甲科成虫食用昆虫的成分、抗氧化和抗菌活性的比较研究。


就拟步甲科昆虫而言，研究集中在幼虫阶段，缺乏其他阶段的信息。因此，进行这项研究是为了了解该科的两个物种在成年阶段以特定饮食喂养时的营养成分和生物活性之间的差异。将两种物种的成年甲虫脱脂、冻干并用缓冲液提取蛋白质。对每种昆虫的提取物进行近端和植物化学分析，并通过 Tris-Tricine 和 Tris Glycine SDS PAGE 进行蛋白质提取物和水解分析。该分析表明，鼹鼠比 U. dermestoides 含有更多的蛋白质和脂肪，但含有更少的粗纤维。使用 PBS 进行蛋白质提取，其中 130 和 45 kDa 条带显示皮纹 U. dermestoides 占主导地位，而 T. molitor 的蛋白质含量较少。测定了酶促蛋白质水解产物和蛋白质粗提物的抗氧化和抗菌活性。在这两种昆虫中都发现了与抗氧化活性相关的蛋白质的存在。尽管如此，含有蛋白质提取物的皮纹 U. dermestoides 具有较高的抗氧化活性，而提取物被消化后，皮纹 U. dermestoides 表现出较高的抗氧化活性。蛋白水解后，蛋白质提取物显示出增强的抗氧化活性，此外还具有抑制变形杆菌、志贺氏菌和芽孢杆菌等微生物生长的能力。昆虫蛋白与蛋白酶的水解物一旦表征，就为使用这些甲虫作为微生物控制的加密肽的新来源提供了可能性。