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Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family.
BMC Chemistry ( IF 4.6 ) Pub Date : 2020-09-09 , DOI: 10.1186/s13065-020-00707-0
Daniel R Flores 1 , Luz E Casados 2 , Sandra F Velasco 1 , Ana C Ramírez 1 , Gilberto Velázquez 1
Affiliation  

In the case of Tenebrionidae family insects, studies focus on larval stage, leaving a lack of information regarding other stages. Therefore, this study was performed in order to understand the differences between the nutritional composition and the bioactivity of two species of this family in their adult stage, fed with a specific diet. Adult beetles of both species were defatted, lyophilized and protein extracted with buffer. Proximal and phytochemical analysis of the extracts of each insect were performed, along with protein extract and hydrolysis analysis by Tris-Tricine and Tris Glycine SDS PAGE. This analysis showed that T. molitor contained more protein and fat than U. dermestoides but contained less crude fiber. The protein extraction was made with PBS, where 130 and 45 kDa bands showed predominant for U. dermestoides, and less protein was present for T. molitor. Antioxidant and antimicrobial activities of the enzymatic protein hydrolysates and protein crude extracts were determined. Presence of protein associated with the antioxidant activity were found in both insects. Nonetheless U. dermestoides had a higher antioxidant activity with the protein extract in contrast with the higher antioxidant activity shown by U. dermestoides once the extracts were digested. After proteolysis, protein extracts showed an increasing antioxidant activity, plus, the ability to inhibit microbial growth of Proteus, Shigella and Bacillus. Insect protein hydrolysates with protease open the possibility for the use of these beetles as new sources of encrypted peptides for microbiological control once characterized.

中文翻译:

两种来自Tenebrionidae科的成年食用昆虫的组成,抗氧化剂和抗菌活性的比较研究。

就天牛科昆虫而言,研究集中在幼虫阶段,而缺乏有关其他阶段的信息。因此,进行这项研究是为了了解该族的两个物种在成年阶段的营养组成和生物活性之间的差异,这些物种饲喂特定饮食。将两种物种的成年甲虫脱脂,冻干并用缓冲液提取蛋白质。对每种昆虫的提取物进行近邻和植物化学分析,并通过Tris-Tricine和Tris Glycine SDS PAGE进行蛋白质提取和水解分析。该分析表明,T。molitor比U. dermestoides含有更多的蛋白质和脂肪,但含有较少的粗纤维。用PBS提取蛋白质,其中130 kDa和45 kDa的条带显示出U. dermestoides,而对于T. molitor存在较少的蛋白质。测定了酶促蛋白水解物和蛋白粗提物的抗氧化和抗菌活性。在两种昆虫中都发现了与抗氧化活性有关的蛋白质。但是,与提取物被消化后的美德美表现出更高的抗氧化活性相反,美德美对蛋白质提取物具有更高的抗氧化活性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌的微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦表征的用于微生物控制的加密肽的新来源的可能性。测定了酶促蛋白水解物和蛋白粗提物的抗氧化和抗菌活性。在两种昆虫中都发现了与抗氧化活性相关的蛋白质。但是,与提取物被消化后的美德美表现出更高的抗氧化活性相反,美德美对蛋白质提取物具有更高的抗氧化活性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌的微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦被表征就可作为微生物控制的加密肽的新来源的可能性。测定了酶促蛋白水解物和蛋白粗提物的抗氧化和抗菌活性。在两种昆虫中都发现了与抗氧化活性有关的蛋白质。但是,与提取物被消化后的美德美表现出更高的抗氧化活性相反,美德美对蛋白质提取物具有更高的抗氧化活性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌的微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦表征的用于微生物控制的加密肽的新来源的可能性。在两种昆虫中都发现了与抗氧化活性有关的蛋白质。然而,与提取物被消化后的美德美表现出更高的抗氧化活性相反,美德美对蛋白质提取物具有更高的抗氧化活性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦被表征就可作为微生物控制的加密肽的新来源的可能性。在两种昆虫中都发现了与抗氧化活性有关的蛋白质。但是,与提取物被消化后的美德美表现出更高的抗氧化活性相反,美德美对蛋白质提取物具有更高的抗氧化活性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌的微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦表征的用于微生物控制的加密肽的新来源的可能性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌的微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦表征的用于微生物控制的加密肽的新来源的可能性。蛋白水解后,蛋白质提取物显示出增加的抗氧化活性,以及​​抑制变形杆菌,志贺氏菌和芽孢杆菌微生物生长的能力。昆虫蛋白与蛋白酶的水解产物打开了将这些甲虫用作一旦被表征就可作为微生物控制的加密肽的新来源的可能性。
更新日期:2020-09-10
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