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Nontarget Screening and Toxicity Evaluation of Diol Esters of Okadaic Acid and Dinophysistoxins Reveal Intraspecies Difference of Prorocentrum lima.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-09-09 , DOI: 10.1021/acs.est.0c03691 Haiyan Wu 1 , Jiaqi Chen 1 , Jixing Peng 1 , Yun Zhong 1 , Guanchao Zheng 1 , Mengmeng Guo 1 , Zhijun Tan 1, 2 , Yuxiu Zhai 1, 2 , Songhui Lu 3
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-09-09 , DOI: 10.1021/acs.est.0c03691 Haiyan Wu 1 , Jiaqi Chen 1 , Jixing Peng 1 , Yun Zhong 1 , Guanchao Zheng 1 , Mengmeng Guo 1 , Zhijun Tan 1, 2 , Yuxiu Zhai 1, 2 , Songhui Lu 3
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High-resolution mass spectrometry (HRMS) analysis with the assistance of molecular networking was used to investigate intracellular toxin profiles of five Prorocentrum lima (P. lima) strains sampled from the north Yellow Sea and South China Sea. Mice were used as a model species for testing the acute toxicity of intracellular okadaic acid (OA) and dinophysistoxins (DTXs) in free and esterified states. Results showed that OA and DTX1 esterified derivatives were detected in all P. lima samples, accounting for 55%–96% of total toxins in five strains. A total of 24 esters and 1 stereoisomer of DTX1 (35S DTX1) were identified based on molecular networking and MS data analysis, 15 esters of which have been reported first. All P. lima strains displayed specific toxin profiles, and preliminary analysis suggested that toxin profiles of the five P. lima strains might be region-related. Moreover, acute toxicity in mice suggested higher toxicity of esters compared with free toxins, which highlights the importance and urgency of attention to esterified toxins in P. lima.
中文翻译：
冈田酸二醇酯和甲藻毒素的非靶点筛选和毒性评价揭示了利马原核的种内差异。
在分子网络的帮助下，高分辨率质谱 (HRMS) 分析用于研究从北黄海和南海取样的五种Prorocentrum lima ( P. lima ) 菌株的细胞内毒素谱。小鼠被用作模型物种，用于测试游离和酯化状态下的细胞内冈田酸 (OA) 和甲藻毒素 (DTX) 的急性毒性。结果表明，在所有P. lima样品中均检测到 OA 和 DTX1 酯化衍生物，占 5 个菌株总毒素的 55%–96%。基于分子网络和MS数据分析，共鉴定出24种酯类和1种DTX1立体异构体（35S DTX1），其中15种酯类首次报道。所有P. lima菌株显示出特定的毒素谱，初步分析表明，五种P. lima菌株的毒素谱可能与区域相关。此外，小鼠的急性毒性表明与游离毒素相比，酯的毒性更高，这突出了关注P. lima中酯化毒素的重要性和紧迫性。
更新日期：2020-10-06
中文翻译：
冈田酸二醇酯和甲藻毒素的非靶点筛选和毒性评价揭示了利马原核的种内差异。
在分子网络的帮助下，高分辨率质谱 (HRMS) 分析用于研究从北黄海和南海取样的五种Prorocentrum lima ( P. lima ) 菌株的细胞内毒素谱。小鼠被用作模型物种，用于测试游离和酯化状态下的细胞内冈田酸 (OA) 和甲藻毒素 (DTX) 的急性毒性。结果表明，在所有P. lima样品中均检测到 OA 和 DTX1 酯化衍生物，占 5 个菌株总毒素的 55%–96%。基于分子网络和MS数据分析，共鉴定出24种酯类和1种DTX1立体异构体（35S DTX1），其中15种酯类首次报道。所有P. lima菌株显示出特定的毒素谱，初步分析表明，五种P. lima菌株的毒素谱可能与区域相关。此外，小鼠的急性毒性表明与游离毒素相比，酯的毒性更高，这突出了关注P. lima中酯化毒素的重要性和紧迫性。
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