Background. Interleukin-17 plays important roles in allergic diseases. Several studies proved that leptin promoted Th17 immune responses by inducing RORγt transcription. ILC2 is an important member of the early stage of immune response. Therefore, we aimed to explore the effect of leptin on the IL-17 production by ILC2 in AR in this study. Methods. Fifteen AR patients and fifteen healthy controls were enrolled. Serum leptin levels were measured, and their correlation with the frequency of IL-17+ ILC2 cells was analyzed using enzyme-linked immunosorbent assay (ELISA) and flow cytometry. ILC2 was stimulated by leptin, and the expression of IL-17, IL-5, and IL-13 was detected by ELISA. The correlated pathways were confirmed by real-time PCR. Results. We found that serum leptin and the frequency of IL-17-producing ILC2s in AR were significantly higher compared with those in controls. After being incubated with leptin, the frequency of IL-17+ ILC2 cells and IL-17 production from ILC2 was upregulated compared with that in controls. We also found that leptin induced RORγt and Ahr expression by ILC2. Moreover, leptin-induced IL-17-producing ILC2 concomitantly expressed IL-5 and IL-13. Conclusions. Our data provide preliminary evidence that leptin-induced IL-17 production from ILC2 cells is dependent on RORγt and Ahr expression and the blockade of leptin may be a promising target for the treatment of AR.

中文翻译：

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瘦素促进过敏性鼻炎中 ILC2 产生 IL-17。

背景。白细胞介素17在过敏性疾病中起重要作用。多项研究证明，瘦素通过诱导 ROR γ t 转录来促进 Th17 免疫反应。ILC2是免疫反应早期的重要成员。因此，本研究旨在探讨瘦素对 AR 中 ILC2 产生 IL-17 的影响。方法。招募了 15 名 AR 患者和 15 名健康对照。测量血清瘦素水平，并使用酶联免疫吸附测定 (ELISA) 和流式细胞术分析其与 IL-17+ ILC2 细胞频率的相关性。瘦素刺激ILC2，ELISA检测IL-17、IL-5、IL-13的表达。通过实时PCR证实了相关途径。结果. 我们发现，与对照组相比，AR 中血清瘦素和产生 IL-17 的 ILC2 的频率显着更高。与瘦素一起孵育后，与对照组相比，IL-17+ ILC2 细胞和 ILC2 产生 IL-17 的频率上调。我们还发现瘦素通过 ILC2诱导 ROR γ t 和 Ahr 表达。此外，瘦素诱导的产生IL-17的ILC2同时表达IL-5和IL-13。结论。我们的数据提供了初步证据，表明瘦素诱导的 ILC2 细胞产生的 IL-17 依赖于 ROR γ t 和 Ahr 表达，而瘦素的阻断可能是治疗 AR 的有希望的靶点。