This paper presents a rapid, sensitive and precise method developed and validated for the quantification of sufentanil in biological samples using ultra-performance liquid chromatography coupled with QqQ-MS-MS. Plasma samples were extracted with simple and fast liquid-liquid extraction (ethyl acetate, pH 9). Calibration curve showed linearity in the concentration range of 0.005–30 µg/L. The lower limit of quantification was 0.010 µg/L. The most important method features are low lower limit of quantification value, simple plasma extraction and small sample volume. This method is suitable not only for evaluation of the pharmacokinetics, toxicology, bioavailability and clinical pharmacology of sufentanil but also for the detection and identification of this compound in human plasma samples for forensic purposes.

中文翻译：

---

UHPLC-QqQ-MS-MS 快速测定人血浆中的舒芬太尼

本文介绍了一种使用超高效液相色谱和 QqQ-MS-MS 对生物样品中的舒芬太尼进行定量分析的快速、灵敏和精确的方法。血浆样品通过简单快速的液-液萃取（乙酸乙酯，pH 9）进行萃取。校准曲线在 0.005–30 µg/L 的浓度范围内呈线性。定量下限为 0.010 µg/L。最重要的方法特点是定量值下限低、血浆提取简单、样品量小。该方法不仅适用于评价舒芬太尼的药代动力学、毒理学、生物利用度和临床药理学，而且适用于法医目的的人血浆样品中该化合物的检测和鉴定。