当前位置：
X-MOL 学术
›
ACS Biomater. Sci. Eng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Magnesium-Based Whitlockite Bone Mineral Promotes Neural and Osteogenic Activities
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-08 , DOI: 10.1021/acsbiomaterials.0c00852 Yafeng Yang 1 , Huadong Wang 1 , Huazhe Yang 2 , Yantao Zhao 1, 3 , Jidong Guo 1 , Xin Yin 1 , Teng Ma 4 , Xiao Liu 5 , Li Li 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-08 , DOI: 10.1021/acsbiomaterials.0c00852 Yafeng Yang 1 , Huadong Wang 1 , Huazhe Yang 2 , Yantao Zhao 1, 3 , Jidong Guo 1 , Xin Yin 1 , Teng Ma 4 , Xiao Liu 5 , Li Li 1
Affiliation
|
Nerves spread throughout human bone minerals and play an important role in regulating osteogenic homeostasis. However, whether the distributive nerves can sense bone minerals and the role of bone minerals in nerve outgrowth are still unclear. We hypothesized that a natural magnesium-containing bone mineral, whitlockite (WH), the second most abundant bone mineral in the human body, could simultaneously promote both osteogenic and neural activities. To verify the hypothesis, both WH and hydroxyapatite (HAP) nanoparticles were synthesized, and their characterization was completed by Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The effect of WH on neural differentiation of mesenchymal stem cells (MSCs) and neural progenitor cells (NPCs) in 2D and 3D culture was examined by immunostaining and quantitative real-time polymerase chain reaction (qRT-PCR). The secretion of calcitonin gene-related polypeptide (CGRP) was examined by enzyme-linked immunosorbent assay (ELISA). The neural and osteogenic differentiation in a preosteoblasts and NPCs coculture system was examined by immunostaining and qRT-PCR. The results showed that WH promotes the gene and protein expression of neuronal specific marker (MAP-2 and βIII-tubulin) in 2D and 3D culture systems. In addition, the neurite length in the WH group was significantly longer than in other groups. Furthermore, both neural differentiation and osteogenic differentiation were simultaneously enhanced in the WH group in the coculture system. Thus, this study demonstrated the simultaneous stimulatory effect of WH bone mineral on neural and osteogenic activities, which provided WH as a valuable material for bone regeneration.
中文翻译：
镁基硅藻土骨矿物质促进神经和成骨活性
神经遍布人的骨矿物质，并在调节成骨稳态中起重要作用。但是，分布神经是否可以感知骨矿物质以及骨矿物质在神经长大中的作用仍不清楚。我们假设一种天然的含镁骨矿物质-辉锰矿（WH），是人体中第二丰富的骨矿物质，可以同时促进成骨和神经活动。为了验证该假设，合成了WH和羟基磷灰石（HAP）纳米粒子，并通过傅立叶变换红外光谱（FT-IR）和X射线衍射（XRD）对其进行了表征。通过免疫染色和定量实时聚合酶链反应（qRT-PCR），检查了WH对2D和3D培养中的间充质干细胞（MSC）和神经祖细胞（NPC）神经分化的影响。降钙素基因相关多肽（CGRP）的分泌通过酶联免疫吸附测定（ELISA）进行检查。通过免疫染色和qRT-PCR检查了成骨细胞和NPC共培养系统中神经和成骨的分化。结果表明，WH可促进2D和3D培养系统中神经元特异性标记（MAP-2和βIII-微管蛋白）的基因和蛋白质表达。此外，WH组的神经突长度明显长于其他组。此外，在共培养系统中，WH组的神经分化和成骨分化均同时增强。因此，这项研究证明了WH骨矿物质对神经和成骨活动的同时刺激作用，这为WH提供了有价值的骨再生材料。
更新日期：2020-10-12
中文翻译：
镁基硅藻土骨矿物质促进神经和成骨活性
神经遍布人的骨矿物质，并在调节成骨稳态中起重要作用。但是，分布神经是否可以感知骨矿物质以及骨矿物质在神经长大中的作用仍不清楚。我们假设一种天然的含镁骨矿物质-辉锰矿（WH），是人体中第二丰富的骨矿物质，可以同时促进成骨和神经活动。为了验证该假设，合成了WH和羟基磷灰石（HAP）纳米粒子，并通过傅立叶变换红外光谱（FT-IR）和X射线衍射（XRD）对其进行了表征。通过免疫染色和定量实时聚合酶链反应（qRT-PCR），检查了WH对2D和3D培养中的间充质干细胞（MSC）和神经祖细胞（NPC）神经分化的影响。降钙素基因相关多肽（CGRP）的分泌通过酶联免疫吸附测定（ELISA）进行检查。通过免疫染色和qRT-PCR检查了成骨细胞和NPC共培养系统中神经和成骨的分化。结果表明，WH可促进2D和3D培养系统中神经元特异性标记（MAP-2和βIII-微管蛋白）的基因和蛋白质表达。此外，WH组的神经突长度明显长于其他组。此外，在共培养系统中，WH组的神经分化和成骨分化均同时增强。因此，这项研究证明了WH骨矿物质对神经和成骨活动的同时刺激作用，这为WH提供了有价值的骨再生材料。
京公网安备 11010802027423号