Dual Therapeutic Targeting of Lung Infection and Carcinoma Using Lactoferrin-Based Green Nanomedicine,ACS Biomaterials Science & Engineering

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Dual Therapeutic Targeting of Lung Infection and Carcinoma Using Lactoferrin-Based Green Nanomedicine
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-08 , DOI: 10.1021/acsbiomaterials.0c01095
Marian G. Rofeal _{1,

2} , Ahmed O. Elzoghby _{2,

3} , Maged W. Helmy _{2,

4} , Rowaida Khalil ₁ , Heba Khairy ₁ , Sanaa Omar ₁

Affiliation

In view of the promising applications of nanoparticles in drug delivery, this study highlights the fabrication of new bioactive green protein–polysaccharide nanocomplexes with significant antibacterial and antitumor efficacies. We preformulated the water-insoluble drugs Quercetin (Quer) and Resveratrol (Res) as water-soluble nanocrystals to facilitate their entrapment in the electrostatic lactoferrin–chondroitin (Lf-ChS) nanocomplex. Quer and Res were physically entrapped in the Lf-ChS nanomatrix with high encapsulation efficiencies (EE %) of 85.2 and 90.1% w/w for Quer and Res, respectively. The in vitro synergetic antibacterial effects of the studied compounds against all bacterial strains were confirmed. Res-Quer Lf-ChS NPs revealed an enhanced cytotoxic effect against A549 lung cancer cells. A new model of polymicrobial lung infection was designed, where treatment with Res-Quer Lf-ChS NPs (233.5 ± 6.59 nm) resulted in a marked decline of 3.2 log units in bacterial counts. In the lung tumor model, the potent antitumor efficacy of the developed Res-Quer Lf-ChS NPs was demonstrated by a noticeable decline in both lung weight and the biomarkers compared to the positive control group that did not receive any treatment. In conclusion, the green Res-Quer Lf-ChS NPs possess antibacterial and antitumor attributes for potential lung infection and tumor therapy.

中文翻译：

基于乳铁蛋白的绿色纳米药物对肺部感染和癌的双重治疗靶向

鉴于纳米颗粒在药物输送中的应用前景广阔，本研究着重介绍了具有显着抗菌和抗肿瘤功效的新型生物活性绿色蛋白质-多糖纳米复合物的制备。我们将水不溶性药物槲皮素（Quer）和白藜芦醇（Res）预先配制为水溶性纳米晶体，以促进它们被静电乳铁蛋白-软骨素（Lf-ChS）纳米复合物截留。Quer和Res被物理包裹在Lf-ChS纳米矩阵中，对Quer和Res的包封效率（EE％）分别为85.2和90.1％w / w。在体外证实了所研究化合物对所有细菌菌株的协同抗菌作用。Res-Quer Lf-ChS NP显示出针对A549肺癌细胞的增强的细胞毒性作用。设计了一种新的多微生物肺部感染模型，其中用Res-Quer Lf-ChS NP（233.5±6.59 nm）处理导致细菌计数显着下降3.2 log单位。在肺肿瘤模型中，与未接受任何治疗的阳性对照组相比，肺重量和生物标志物均明显下降，从而证明了已开发的Res-Quer Lf-ChS NP具有很强的抗肿瘤功效。总之，绿色的Res-Quer Lf-ChS NP具有潜在的肺部感染和肿瘤治疗的抗菌和抗肿瘤特性。

更新日期：2020-10-12

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