ABSTRACT Heat stress is a major growth-limiting factor for most crops over the world. Chitin elicitor receptor kinase 1 (CERK1) is a chitin/chitooligosaccharides receptor, and ERECTA (ER) plays a crucial role in plant resistance to heat stress. In the present study, a chitooligosaccharides-induced CERK1n-ERc fusion gene was designed and synthesized, in which the extracellular domain and transmembrane domain of CERK1 gene is connected with the response region of ER gene. We successfully constructed the CERK1n-ERc fusion gene by Overlap PCR and introduced it into Arabidopsis by Agrobacterium-medicated infection. Genetically modified (GM) plants had a greater germination rate and germination index, as well as a shorter mean germination time, indicating that they had a better thermotolerance compared with the wild-type (WT) lines under heat stress. Moreover, the GM lines showed a lower level of hydrogen peroxide (H2O2) and relative electrolyte leakage (REL), suggesting that they were in better state compared with the WT plants when exposed to high temperature. UPLC-MS/MS was employed to assess the phytohormone level, suggesting that the GM lines acquired a better thermotolerance via jasmonic acid (JA) signaling pathways. In general, we constructed a COS-induced fusion gene to enhance the thermotolerance of Arabidopsis during seed germination and postgermination growth.

中文翻译：

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壳寡糖诱导的 CERK1n-ERc 融合基因增强拟南芥的耐热性

摘要 热应激是世界上大多数作物的主要生长限制因素。几丁质激发子受体激酶 1 (CERK1) 是一种几丁质/壳寡糖受体，ERECTA (ER) 在植物对热胁迫的抗性中起着至关重要的作用。本研究设计合成了壳寡糖诱导的CERK1n-ERc融合基因，其中CERK1基因的胞外域和跨膜域与ER基因的反应区相连。我们通过重叠PCR成功构建了CERK1n-ERc融合基因，并通过农杆菌介导感染将其导入拟南芥。转基因 (GM) 植物具有更高的发芽率和发芽指数，以及更短的平均发芽时间，表明它们与热应激下的野生型 (WT) 品系相比具有更好的耐热性。此外，转基因株系显示出较低水平的过氧化氢 (H2O2) 和相对电解质泄漏 (REL)，表明它们在暴露于高温时与 WT 植物相比处于更好的状态。UPLC-MS/MS 用于评估植物激素水平，表明转基因品系通过茉莉酸 (JA) 信号通路获得了更好的耐热性。总的来说，我们构建了一个 COS 诱导的融合基因，以增强拟南芥在种子萌发和萌发后生长过程中的耐热性。表明转基因品系通过茉莉酸 (JA) 信号通路获得了更好的耐热性。总的来说，我们构建了一个 COS 诱导的融合基因，以增强拟南芥在种子萌发和萌发后生长过程中的耐热性。表明转基因品系通过茉莉酸 (JA) 信号通路获得了更好的耐热性。总的来说，我们构建了一个 COS 诱导的融合基因，以增强拟南芥在种子萌发和萌发后生长过程中的耐热性。