Abstract

Background and purpose

Huntington disease (HD) is an autosomal dominant inheritance neurodegenerative disorder. 3-Nitropropanoic acid (3-NP) is a mitochondrial toxin that induces HD-like symptoms and thus serves as a good experimental model of HD. Chrysin (5, 7-dihydroxyflavone) is a natural flavonoid that have multiple biological activities. The present work was aimed to evaluate the neuroprotective efficacy of Chrysin in rat brain, under the influence of 3-NP treatment, by studying neurobehavioral and biochemical alterations alongwith histo-architectural changes.

Materials and methods

Male Wistar rats (220–250 g) were used in the study and were divided into three groups following randomization. Each group comprised of nine animals. Group I animals served as control group and administered with normal saline (orally) as vehicle. Animals of Group II were treated with 3-NP for four successive days, at the dose of 20 mg/kg, intraperitoneally (i.p.). Animals that received Chrysin for the period of four consecutive days with the dose of 50 mg/kg, orally twice daily (30 min pre-treatment and 6 h post-treatment) following 3-NP administration served as Group III. After the treatment regime, animals were evaluated for neurobehavioral alterations and brain homogenates were used for estimation of neurotoxicity marker activity and neurotransmitter level along with histological assessment.

Results

The significant alteration in neurobehavioral, biochemical and neuronal structure in striatal part of brain was observed in the 3-NP administered (Group II) animals. It was observed that co-treatment of Chrysin with 3-NP treated rats the rotarod performance, grip strength, stride length as well as monoamine oxidase activity and serotonin levels were elevated.

Conclusion

The results of this study reveal that Chrysin treatment alleviated the neurobehavioral, biochemical and histological alterations against HD symptoms in rats.

中文翻译：

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白杨素可改善 3 种硝基丙酸诱导的针对行为、生化和组织学改变的神经毒性

摘要

背景和目的

亨廷顿病 (HD) 是一种常染色体显性遗传的神经退行性疾病。3-硝基丙酸 (3-NP) 是一种线粒体毒素，可诱导 HD 样症状，因此可作为 HD 的良好实验模型。Chrysin (5, 7-dihydroxyflavone) 是一种具有多种生物活性的天然类黄酮。目前的工作旨在通过研究神经行为和生化变化以及组织结构变化来评估白杨素在 3-NP 治疗的影响下对大鼠大脑的神经保护作用。

材料和方法

雄性 Wistar 大鼠 (220-250 g) 用于研究，并在随机化后分为三组。每组由九只动物组成。I组动物作为对照组并用生理盐水（口服）作为载体给药。第 II 组的动物用 3-NP 连续 4 天治疗，剂量为 20 mg/kg，腹膜内 (ip)。在 3-NP 给药后以 50 mg/kg 剂量每天口服两次（治疗前 30 分钟和治疗后 6 小时）连续四天接受白杨素的动物作为第 III 组。在治疗方案之后，评估动物的神经行为改变，并将脑匀浆用于估计神经毒性标志物活性和神经递质水平以及组织学评估。

结果

在 3-NP 给药 (II 组) 动物中观察到大脑纹状体部分的神经行为、生化和神经元结构的显着改变。观察到白杨素与 3-NP 处理的大鼠共同处理转棒性能、握力、步幅以及单胺氧化酶活性和血清素水平升高。

结论

这项研究的结果表明，白杨素治疗减轻了大鼠 HD 症状的神经行为、生化和组织学改变。