ABSTRACT

1. Salmonella Gallinarum (SG) infections cause fowl typhoid, which leads to important economic losses. Multidrug resistance (MDR) and the capacity for bacteria to form biofilms could play an important role in the persistence of SG in poultry flocks resulting in intermittent disease outbreaks. The aim of the following study was to assess the lytic activity of two new bacteriophages (Salmonella phages UPF_BP1 and UPF_BP2) against MDR and biofilm-forming SG.

2. Forty-six strains of SG, isolated in 2015, were characterised by antimicrobial resistance, biofilm formation profiles and susceptibility to two new bacteriophages.

3. Of these strains, 24% were multidrug resistant and more than 80% formed biofilm, with no statistical difference between incubation temperatures (42°C or 22°C). With regard to the lytic activity of the phages, 85% of strains were susceptible to at least one phage. Of these, 74% were lysed by both phages, including MDR and biofilm producing strains.

4. The use of salmonella phages UPF_BP1 and UPF_BP2 were shown to be promising alternatives for the biological control of fowl typhoid.

摘要

1. 鸡沙门氏菌（SG）感染会导致禽类伤寒，从而导致重大经济损失。多药耐药性（MDR）和细菌形成生物膜的能力可能对家禽群中SG的持久性起重要作用，导致间歇性疾病暴发。以下研究的目的是评估两种新噬菌体（沙门氏菌噬菌体UPF_BP1和UPF_BP2）对MDR和形成生物膜的SG的裂解活性。

2. 2015年分离出的46株SG菌株具有抗药性，生物膜形成特征和对两种新噬菌体的敏感性。

3.在这些菌株中，有24％具有多重耐药性，并且形成的生物膜超过80％，孵育温度（42°C或22°C）之间无统计学差异。关于噬菌体的裂解活性，85％的菌株对至少一种噬菌体敏感。其中，有74％的噬菌体（包括MDR和产生生物膜的菌株）裂解了。

4.沙门氏菌噬菌体UPF_BP1和UPF_BP2的使用被证明是禽类伤寒生物防治的有前途的替代方法。