Abstract

This study aimed to synthesize zinc nanoparticles with antimicrobial and antioxidant activities using alternan (ZnNPs-Alt) produced by Lactobacillus reuteri E81 as the stabilizing agent. For the characterization of the ZnNPs-Alt, UV–vis spectroscopy, SEM, TEM and EDX analysis, XRD, FTIR and DSC analysis were applied. The functional role of ZnNPs-Alt was tested by determination of their antioxidant activities by DPPH and CUPRAC methodologies and their antibacterial and antifungal activities. Results of this study demonstrated that alternan was utilized as a successful stabilizer to produce crystalline, thermally stabile ZnNPs-Alt with a particle size of ∼ 100 nm. Importantly, strong antioxidant and antimicrobial activities were observed for ZnNPs-Alt in a concentration dependent manner. These findings confirmed the role of alternan as a stabilizing agent for the production of ZnNPs-Alt with functional roles.

摘要

这项研究的目的是使用罗伊氏乳杆菌生产的alternan（ZnNPs-Alt）合成具有抗菌和抗氧化活性的锌纳米颗粒。E81作为稳定剂。为了表征ZnNPs-Alt，采用紫外可见光谱，SEM，TEM和EDX分析，XRD，FTIR和DSC分析。ZnNPs-Alt的功能作用通过DPPH和CUPRAC方法确定其抗氧化活性以及其抗菌和抗真菌活性进行了测试。这项研究的结果表明，交替糖被用作成功的稳定剂，以生产粒径约100 nm的结晶，热稳定的ZnNPs-Alt。重要的是，以浓度依赖的方式观察到了ZnNPs-Alt的强抗氧化剂和抗菌活性。这些发现证实了交替糖作为生产具有功能性作用的ZnNPs-Alt的稳定剂的作用。