ABSTRACT Crocodile oil has been used worldwide for cosmetic and pharmaceutical purposes due to its essential fatty acid contents. This study aimed to investigate the best extraction method of crocodile oil extracted from abdominal adipose tissues (Crocodylus siamensis) by using four different ways. The results showed that the oil derived from the dry rendering method had lower levels of omega-3 alpha-linolenic acid (0.92 ± 0.049 g per 100 g sample) when compared to the oil extracted by using the other methods (1.16 ± 0.142, 0.96 ± 0.049, and 1.18 ± 0.010 g per 100 g sample, respectively). Interestingly, the crocodile oil extracted by wet cold pressing showed no bottom clouded sediment with a more transparent light-yellow color compared to the other oil samples. Moreover, the principle component analysis plots showed a highly positive relationship (r > 0.87) for docosahexaenoic acid (omega-3) and arachidonic acid (omega-6) in crocodile oil extracted using all methods. Therefore, it could be suggested that the modified wet cold pressing method could produce high-quality crocodile oil and could be adapted to further food and dietary supplement processing industries.

中文翻译：

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不同提取方法提取的鳄鱼油(Crocodylus siamensis)的理化性质和脂肪酸组成比较

摘要 鳄鱼油因其必需脂肪酸含量而在世界范围内用于化妆品和药物用途。本研究旨在探讨从腹部脂肪组织 (Crocodylus siamensis) 中提取鳄鱼油的最佳提取方法，采用四种不同的方法。结果表明，与使用其他方法提取的油（1.16 ± 0.142、0.96）相比，采用干法提炼的油具有较低水平的 omega-3 α-亚麻酸（每 100 克样品 0.92 ± 0.049 克） ± 0.049 和 1.18 ± 0.010 g 每 100 g 样品）。有趣的是，与其他油样相比，通过湿冷压榨提取的鳄鱼油没有底部混浊的沉积物，而是更透明的浅黄色。而且，主成分分析图显示，使用所有方法提取的鳄鱼油中的二十二碳六烯酸 (omega-3) 和花生四烯酸 (omega-6) 呈高度正相关 (r > 0.87)。因此，可以建议改进的湿冷压方法可以生产高质量的鳄鱼油，并且可以适用于进一步的食品和膳食补充剂加工业。