Multiple epidemiological studies identify Dolosigranulum pigrum as a candidate beneficial bacterium based on its positive association with health, including negative associations with nasal/nasopharyngeal colonization by the pathogenic species Staphylococcus aureus and Streptococcus pneumoniae. Using a multipronged approach to gain new insights into D. pigrum function, we observed phenotypic interactions and predictions of genomic capacity that support the idea of a role for microbe-microbe interactions involving D. pigrum in shaping the composition of human nasal microbiota. We identified in vivo community-level and in vitro phenotypic cooperation by specific nasal Corynebacterium species. Also, D. pigrum inhibited S. aureus growth in vitro, whereas robust inhibition of S. pneumoniae required both D. pigrum and a nasal Corynebacterium together. D. pigrum l-lactic acid production was insufficient to account for these inhibitions. Genomic analysis of 11 strains revealed that D. pigrum has a small genome (average 1.86 Mb) and multiple predicted auxotrophies consistent with D. pigrum relying on its human host and on cocolonizing bacteria for key nutrients. Further, the accessory genome of D. pigrum harbored a diverse repertoire of biosynthetic gene clusters, some of which may have a role in microbe-microbe interactions. These new insights into D. pigrum’s functions advance the field from compositional analysis to genomic and phenotypic experimentation on a potentially beneficial bacterial resident of the human upper respiratory tract and lay the foundation for future animal and clinical experiments.

中文翻译：

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Dolosigranulum pigrum 在人类鼻腔微生物群中的合作与竞争。

多项流行病学研究将Dolosigranulum pigrum确定为候选有益细菌，因为它与健康呈正相关，包括与致病菌金黄色葡萄球菌和肺炎链球菌在鼻/鼻咽部定植的负相关。使用多管齐下的方法获得对D. pigrum功能的新见解，我们观察了表型相互作用和基因组容量的预测，支持涉及D. pigrum 的微生物-微生物相互作用在塑造人类鼻腔微生物群组成中的作用的想法。我们确定了体内社区水平和体外特定鼻棒状杆菌属物种的表型合作。此外，D. pigrum在体外抑制了金黄色葡萄球菌的生长，而对肺炎链球菌的强烈抑制需要D. pigrum和鼻棒杆菌。D. pigrum l-乳酸的产生不足以解释这些抑制作用。对 11 个菌株的基因组分析表明，猪瘟的基因组较小（平均 1.86 Mb）和多种预测的营养缺陷型，这与猪猪依赖其人类宿主和共聚细菌获取关键营养物一致。此外，辅助基因组 D. pigrum拥有多种生物合成基因簇，其中一些可能在微生物-微生物相互作用中起作用。这些对D. pigrum功能的新见解推动了该领域从组成分析到人类上呼吸道潜在有益细菌的基因组和表型实验，并为未来的动物和临床实验奠定了基础。