Diego Huet studies the organelles involved in the metabolic adaptations of the apicomplexan parasite Toxoplasma gondii. In this mSphere of Influence article, he reflects on how the paper “Absolute quantification of matrix metabolites reveals the dynamics of mitochondrial metabolism” by Chen et al. (W. W. Chen, E. Freinkman, T. Wang, K. Birsoy, and D. M. Sabatini, Cell 166:1324–1337.e11, 2016, https://doi.org/10.1016/j.cell.2016.07.040) shaped his research by providing an approach for rapidly and specifically isolating mitochondria to probe the metabolism of these organelles.

中文翻译：

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影响范围：调整细胞器纯化方法。

Diego Huet研究了apicomplexan寄生虫弓形虫的代谢适应中的细胞器。在这篇“影响力领域”文章中，他反思了Chen等人的论文“基质代谢物的绝对定量揭示了线粒体代谢的动力学”。（WW Chen，E。Freinkman，T。Wang，K。Birsoy和DM Sabatini，Cell 166：1324-1337.e11，2016，https：//doi.org/10.1016/j.cell.2016.07.040）他的研究通过提供一种快速而特异性地分离线粒体的方法来探测这些细胞器的代谢。