Capillary electrophoresis‐mass spectrometry (CE‐MS) is now a mature analytical technique in metabolomics, notably for the efficient profiling of polar and charged metabolites. Over the past few years, (further) progress has been made in the design of improved interfacing techniques for coupling CE to MS; also, in the development of CE‐MS approaches for profiling metabolites in volume‐restricted samples, and in strategies that further enhance the metabolic coverage. In this article, which is a follow‐up of a previous review article covering the years 2016–2018 (Electrophoresis 2019, 40, 165–179), the main (technological) developments in CE‐MS methods and strategies for metabolomics are discussed covering the literature from July 2018 to June 2020. Representative examples highlight the utility of CE‐MS in the fields of biomedical, clinical, microbial, plant and food metabolomics. A complete overview of recent CE‐MS‐based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings, and MS detection mode. Finally, some general conclusions and perspectives are given.

中文翻译：

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代谢组学的 CE-MS：2018-2020 年的发展和应用

毛细管电泳质谱（CE-MS）现已成为代谢组学中成熟的分析技术，特别是对于极性和带电代谢物的有效分析。在过去的几年中，CE 与 MS 耦合的改进接口技术的设计取得了（进一步）进展；此外，还开发了用于分析体积受限样品中代谢物的 CE-MS 方法，以及进一步提高代谢覆盖率的策略。本文是之前一篇涵盖 2016-2018 年的综述文章 ( Electrophoresis 2019, 40, 165-179) 的后续文章，讨论了 CE-MS 方法和代谢组学策略的主要（技术）发展，涵盖2018 年 7 月至 2020 年 6 月的文献。代表性示例强调了 CE-MS 在生物医学、临床、微生物、植物和食品代谢组学领域的实用性。表格中给出了最近基于 CE-MS 的代谢组学研究的完整概述，其中提供了有关样品类型和预处理、毛细管涂层和 MS 检测模式的信息。最后，给出了一些一般性结论和观点。