Recently, a corona virus disease (COVID‐19) caused by a novel corona virus (sevier acute respiratory syndrome corona virus 2; SARS‐CoV‐2), rapidly spread throughout the world. It has been resulted an unprecedented public health crisis and has become a global threat. WHO declared it as a pandemic due to rapid transmission and severity of the disease. According to WHO, as of 22nd of August 2020, the disease spread over 213 countries of the world having 22,812,491 confirmed cases and 795,132 deaths recorded worldwide. In the absence of suitable antiviral drugs and vaccines, the current pandemic has created an urgent need for accurate diagnostic tools that would be helpful for early detection of the patients. Many tests including classical and high‐throughput techniques have developed and obtained U.S. Food and drug administration (FDA) approval. However, efforts are being made to develop new diagnostic tools for detection of the disease. Several molecular diagnostic tests such as real‐time‐polymerase chain reaction, real‐time isothermal loop‐mediated amplification (RT‐LAMP), full genome analysis by next‐generation sequencing, clustered regularly interspaced short palindromic repeats technique and microarray‐based assays along with other techniques such as computed tomography scan, biomarkers, biosensor, nanotechnology, serological test, enzyme‐linked immunosorbent assay (ELISA), isolation of viral strain in cell culture are currently available for diagnosis of COVID‐19 infection. This review provides a brief overview of promising high‐throughput techniques currently used for detection of SARS‐CoV‐2, along with their scope and limitations that may be used for effective control of the disease.

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诊断冠状病毒疾病的最新生物技术工具：综述

最近，一种由新型日冕病毒（严重急性呼吸系统综合症冠状病毒2； SARS-CoV-2）引起的日冕病毒疾病（COVID-19）在世界范围内迅速传播。它造成了前所未有的公共卫生危机，并已成为全球性威胁。由于该疾病的快速传播和严重性，世卫组织宣布其为大流行病。根据世界卫生组织的数据，截至2020年8月22日，该病已蔓延至全球213个国家，在世界范围内已确诊22,812,491例确诊病例，死亡795,132例死亡。在缺乏合适的抗病毒药物和疫苗的情况下，当前的大流行迫切需要精确的诊断工具，这些工具将有助于患者的早期发现。已经开发并获得了包括经典和高通量技术在内的许多测试，并获得了美国食品药品监督管理局（FDA）的批准。但是，正在努力开发用于检测该疾病的新的诊断工具。几种分子诊断测试，例如实时聚合酶链反应，实时等温环介导扩增（RT-LAMP），下一代测序的全基因组分析，聚类的规则间隔短回文重复技术和基于微阵列的检测通过计算机断层扫描，生物标志物，生物传感器，纳米技术，血清学检测，酶联免疫吸附测定（ELISA）和细胞培养中病毒株的分离等其他技术，目前可用于诊断COVID-19感染。这篇综述简要概述了目前用于检测SARS-CoV-2的有前途的高通量技术，