Increased survival of the honey bee Apis mellifera infected with the microsporidian Nosema ceranae by effective gene silencing.,Archives of Insect Biochemistry and Physiology

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Increased survival of the honey bee Apis mellifera infected with the microsporidian Nosema ceranae by effective gene silencing.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-09-09 , DOI: 10.1002/arch.21734
In-Hui Kim _{1,

2} , Dong-Jun Kim ₁ , Won-Seok Gwak _{1,

3} , Soo-Dong Woo ₁

Affiliation

This study examined the control of nosemosis caused by Nosema ceranae, one of the hard‐to‐control diseases of honey bees, using RNA interference (RNAi) technology. Double‐stranded RNA (dsRNA) for RNAi application targeted the mitosome‐related genes of N. ceranae. Among the various mitosome‐related genes, NCER_100882, NCER_101456, NCER_100157, and NCER_100686 exhibited relatively low homologies with the orthologs of Apis mellifera. Four gene‐specific dsRNAs were prepared against the target genes and applied to the infected A. mellifera to analyze Nosema proliferation and honey bee survival. Two dsRNAs specifics to NCER_101456 and NCER_100157 showed high inhibitory effects on spore production by exhibiting only 62% and 67%, respectively, compared with the control. In addition, these dsRNA treatments significantly rescued the honey bees from the fatal nosemosis. It was confirmed that the inhibition of Nosema spore proliferation and the increase in the survival rate of honey bees were resulted from a decrease in the expression level of each target gene by dsRNA treatment. However, dsRNA mixture treatment was no more effective than single treatments in the rescue from the nosemosis. It is expected that the four newly identified mitosome‐related target genes in this study can be effectively used for nosemosis control using RNAi technology.

中文翻译：

通过有效的基因沉默，提高了被微孢子虫Nosema ceranae感染的蜜蜂Apis mellifera的存活率。

这项研究使用RNA干扰（RNAi）技术检查了由Nosema ceranae（一种难于控制的蜜蜂疾病）引起的鼻病的控制。用于RNAi的双链RNA（dsRNA）靶向了中华猪笼草的线粒体相关基因。在与线粒体相关的各种基因中，NCER_100882，NCER_101456，NCER_100157和NCER_100686与蜜蜂蜜蜂的直系同源基因表现出相对较低的同源性。针对靶基因准备了四个基因特异性dsRNA，并将其应用于感染的A. mellifera以分析Nosema繁殖和蜜蜂生存。与对照组相比，对NCER_101456和NCER_100157特异的两种dsRNA对孢子产生具有高抑制作用，分别仅表现出62％和67％。另外，这些dsRNA治疗显着地使蜜蜂免于致命的鼻病。证实了抑制Nosema孢子增殖和提高蜜蜂存活率的原因是通过dsRNA处理降低了每个靶基因的表达水平。然而，dsRNA混合液治疗在鼻炎的挽救方面没有比单一治疗有效。预期该研究中的四个新发现的与线粒体相关的靶基因可有效利用RNAi技术用于鼻病控制。

更新日期：2020-11-16

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