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Dual-peak long period fibre grating coated with graphene oxide for label-free and specific assays of H5N1 virus.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-09-09 , DOI: 10.1002/jbio.202000279 Binbin Luo 1 , Zhijiang Liu 1 , Xin Wang 1 , Shenghui Shi 1 , Nianbing Zhong 1 , Peijie Ma 2 , Shengxi Wu 2 , Decao Wu 1 , Mingfu Zhao 1 , Wangwang Liang 3
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-09-09 , DOI: 10.1002/jbio.202000279 Binbin Luo 1 , Zhijiang Liu 1 , Xin Wang 1 , Shenghui Shi 1 , Nianbing Zhong 1 , Peijie Ma 2 , Shengxi Wu 2 , Decao Wu 1 , Mingfu Zhao 1 , Wangwang Liang 3
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Avian influenza is an acute infectious disease caused by the avian influenza virus (AIV), which has caused enormous economic losses and posed considerable threats to public health. This study aimed to demonstrate an immunosensor based on dispersion turning point long‐period fiber grating (DTP‐LPFG) integrated with graphene oxide (GO) for the specific detection of a type of AIV H5N1 virus. LPFG was designed to work at DTP, whose dual‐peak spacing was very high sensitive to a refractive index. Anti‐H5N1 monoclonal antibodies were covalently bonded with the GO film on the fiber surface, thus constructing an immunosensor for the label‐free and specific detection of the H5N1 virus. The proposed method was capable of the reliable detection of H5N1 virus with the limit of detection as low as ~1.05 ng/ml within the large range of 1 ng/mL to 25 µg/mL. More importantly, immunoassays of the whole H5N1 virus in clinical samples further confirmed that the GO‐integrated DTP‐LPFG immunosensor showed very high specificity to the H5N1 virus and demonstrated great potential for clinical use.
中文翻译：
涂有氧化石墨烯的双峰长周期光纤光栅，用于H5N1病毒的无标记和特异性测定。
禽流感是由禽流感病毒（AIV）引起的一种急性传染病，已造成巨大的经济损失，并对公共卫生构成相当大的威胁。这项研究旨在证明一种基于分散转折点长周期光纤光栅（DTP-LPFG）和氧化石墨烯（GO）的免疫传感器，用于特异性检测AIV H5N1型病毒。LPFG设计用于DTP，DTP的双峰间距对折射率非常敏感。抗H5N1单克隆抗体与纤维表面的GO膜共价结合，从而构建了一种用于无标记和特异性检测H5N1病毒的免疫传感器。所提出的方法能够可靠地检测H5N1病毒，在1 ng / mL至25 µg / mL的大范围内，检测限低至〜1.05 ng / ml。
更新日期：2020-09-09
中文翻译：
涂有氧化石墨烯的双峰长周期光纤光栅，用于H5N1病毒的无标记和特异性测定。
禽流感是由禽流感病毒（AIV）引起的一种急性传染病，已造成巨大的经济损失，并对公共卫生构成相当大的威胁。这项研究旨在证明一种基于分散转折点长周期光纤光栅（DTP-LPFG）和氧化石墨烯（GO）的免疫传感器，用于特异性检测AIV H5N1型病毒。LPFG设计用于DTP，DTP的双峰间距对折射率非常敏感。抗H5N1单克隆抗体与纤维表面的GO膜共价结合，从而构建了一种用于无标记和特异性检测H5N1病毒的免疫传感器。所提出的方法能够可靠地检测H5N1病毒，在1 ng / mL至25 µg / mL的大范围内，检测限低至〜1.05 ng / ml。
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