Development of durable resistance to soybean rust (SBR) is challenging due to the pathogenic diversity of Phakopsora pachyrhizi populations. The objective of this research was to investigate and confirm the genomic locations of Rpp genes in the Ugandan line UG‐5 that confer resistance to different SBR pathotypes. Bulked segregant analysis revealed two genomic regions associated with resistance in a cross with rust‐susceptible 'Williams 82'. Composite interval mapping in the F₂ and F_2:3 populations had a LOD score of 48.7 in a region 0.38 cM away from the estimated location of the Rpp1 locus on chromosome (Chr.) 18. An approximately 23‐Kbp interval spanning the Rpp1 locus was flanked by SNP markers ss715632313 and ss715632318. Another interval was identified at the Rpp3 locus on Chr. 6 between markers Satt100 and ss715594488 (2.4 cM) in the F₂ population and between Satt100 and ss715594874 (4.3 cM) in the F_2:3 population, with a maximum LOD score of 25.6. UG‐5 was thus confirmed to have SBR resistance genes at the Rpp1 and Rpp3 loci that can be pyramided into other elite cultivars.

中文翻译：

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大豆品系UG-5（Glycine max）中赋予两个抗大豆锈病基因（Phakopsora pachyrhizi）的定位和确认

由于Phakosora pachyrhizi种群的致病性多样性，发展对大豆锈病（SBR）的持久抗性具有挑战性。这项研究的目的是调查并确认乌干达品系UG-5中Rpp基因的基因组位置，这些基因赋予对不同SBR病态类型的抗性。大量分离剂分析显示，与易受锈病影响的“ Williams 82”杂交的抗性相关的两个基因组区域。F ₂和F _2：3群体中的复合区间作图在距离染色体（Chr。）18上Rpp1基因座的估计位置0.38 cM的区域中的LOD得分为48.7，大约为23 Kbp区间。SNP标记ss715632313和ss715632318位于基因座的两侧。在Chr的Rpp3位点确定了另一个间隔。F ₂群体中的标记Satt100和ss715594488（2.4 cM）之间为6，F _2：3群体中的Satt100和ss715594874（4.3 cM）之间为6，最大LOD得分为25.6。因此，UG-5被证实在Rpp1和Rpp3位点具有SBR抗性基因，可以与其他优良品种金字塔化。