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Expression of sirtuins in ovarian follicles of postnatal mice.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-09-09 , DOI: 10.1002/mrd.23418
Deqi Kong 1, 2 , Guidong Yao 1, 2 , Yucheng Bai 1, 2 , Guang Yang 1, 2 , Ziwen Xu 1, 2 , Yue Kong 1, 2 , Huiying Fan 1, 2 , Qina He 1, 2 , Yingpu Sun 1, 2
Affiliation  

Mammalian ovarian follicular development is an intricate, elaborate, and well‐organized phenomenon regulated by various signaling pathways; however, the underlying mechanism remains unclear. Mammalian sirtuins (sirtuin 1 to sirtuin 7) are a group of NAD+‐dependent deacetylases implicated in various physiological processes including cell proliferation, apoptosis, cell cycle progression, and insulin signaling. Mammalian ovarian sirtuins have been studied using adult and aged bovine, porcine, and murine models. However, limited information is available regarding their precise expression patterns and the localization of follicle development in mice. This study aimed to assess the dynamic expression and localization of all seven sirtuins in early postnatal mouse ovaries through real‐time polymerase chain reaction analysis and immunohistochemistry, respectively. During postnatal ovarian follicle development, sirtuin 1, sirtuin 4, and sirtuin 6 were downregulated compared with those in 1‐day postnatal mouse ovaries (p < .05), indicating that these three sirtuin genes may be markers of follicular development. Combining their localization in granulosa cells through immunohistochemical studies, sirtuin 1, sirtuin 4, and sirtuin 6 are suggested to play negative regulatory roles in mammal ovarian follicular granulosa cell development. Furthermore, we found that sirtuin 2 (p < .05) and sirtuin 7 (p < .05) mRNA were constantly upregulated relative to sirtuin 1, although limited information is available regarding sirtuin 7. Among all sirtuins in mouse ovaries, sirtuin 1 was relatively and steadily downregulated. Upon sirtuin 1 overexpression in 1‐day postnatal mouse ovaries via sirtuin 1‐harboring adenoviruses in vitro, the emergence of primary follicles was delayed, as was the emergence of secondary follicles in 4‐day postnatal ovaries. Further studies on KGN cell lines reported that interfering with sirtuin 1 expression in granulosa cell significantly affected granulosa cell proliferation and the expression of mitochondrial genes. This study presents the first systemic analysis of dynamic patterns of sirtuin family expression in early postnatal mice ovaries, laying the foundation for further studies on less discussed sirtuin subtypes, such as sirtuin 5 and sirtuin 7.

中文翻译:

Sirtuins 在出生后小鼠卵巢卵泡中的表达。

哺乳动物卵巢卵泡发育是一种复杂、精细且组织良好的现象,受各种信号通路调节;然而,潜在的机制仍不清楚。哺乳动物sirtuin(sirtuin 1到sirtuin 7)是一组NAD +依赖的脱乙酰酶参与各种生理过程,包括细胞增殖、凋亡、细胞周期进程和胰岛素信号传导。已经使用成年和老年牛、猪和鼠模型研究了哺乳动物卵巢 Sirtuins。然而,关于它们的精确表达模式和小鼠卵泡发育定位的信息有限。本研究旨在分别通过实时聚合酶链反应分析和免疫组织化学评估所有七种沉默调节蛋白在出生后早期小鼠卵巢中的动态表达和定位。在出生后卵巢卵泡发育过程中,与出生后 1 天的小鼠卵巢相比,sirtuin 1、sirtuin 4 和 Sirtuin 6 下调(p < .05),表明这三个sirtuin基因可能是卵泡发育的标志物。通过免疫组织化学研究结合它们在颗粒细胞中的定位,sirtuin 1、sirtuin 4 和 sirtuin 6 被建议在哺乳动物卵巢滤泡颗粒细胞发育中发挥负调控作用。此外,我们发现sirtuin 2 ( p  < .05) 和sirtuin 7 ( p < .05) mRNA 相对于 sirtuin 1 不断上调,尽管关于 sirtuin 7 的信息有限。在小鼠卵巢中的所有 sirtuin 中,sirtuin 1 相对稳定地下调。通过体外携带sirtuin 1的腺病毒在出生后1天的小鼠卵巢中过度表达sirtuin 1后,初级卵泡的出现被延迟,出生后4天卵巢中次级卵泡的出现也是如此。对 KGN 细胞系的进一步研究报道,干扰颗粒细胞中的 Sirtuin 1 表达显着影响颗粒细胞增殖和线粒体基因的表达。本研究首次对出生后早期小鼠卵巢中 Sirtuin 家族表达的动态模式进行了系统分析,为进一步研究较少讨论的 Sirtuin 亚型奠定了基础,
更新日期:2020-10-30
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