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Scanning electron microscopic evaluation of the efficacy of 5% ethylenediaminetetraacetic acid for smear layer removal.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-09-09 , DOI: 10.1002/jemt.23582 Ekim Onur Orhan 1 , Duygu Bahadır 1 , Ceyda Akgün 1 , Seher Aktaş 1
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-09-09 , DOI: 10.1002/jemt.23582 Ekim Onur Orhan 1 , Duygu Bahadır 1 , Ceyda Akgün 1 , Seher Aktaş 1
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The purpose of the study was to evaluate the smear layer removal efficacy of novel Saver® irrigating solution including 5% EDTA and to compare the 17% EDTA as the most used counterpart. Forty‐four single‐rooted teeth were divided into four groups, were instrumented, and irrigated. The smear treatment groups were as follows: sterile distilled water; 5.25% sodium hypochlorite; Saver®; 17% EDTA. The root samples were blinded. The micrographic images of root samples were collected by scanning electron microscope. The percentages of open dentin tubules were quantified using an image analysis software on micrographs. Two‐way ANOVA followed by Tukey's multiple comparisons was used (p < .05). The 17% EDTA showed the highest percentages of open dentin tubules than the other treatments (p < .01). The mean percentages of open dentin tubules of 17% EDTA revealed statistically highly significant differences than Saver® treatment in all root regions (p < .01). Dentin tubules were covered by dense smear layer in distilled water irrigation whereas, the tubular pattern of dentin structure was not observed in hypochlorite irrigation. The commercial Saver® product presented to be less cleaning property on the smear layer than 17% EDTA and failed to show any improvement in the removal of smear layer compared with its tested counterpart.
中文翻译：
扫描电镜评估5％乙二胺四乙酸去除涂片层的功效。
该研究的目的是评估新颖的Saver®冲洗溶液（包括5％EDTA）的去污层去除功效，并比较17％EDTA作为最常用的冲洗溶液。将四十四根单根牙齿分为四组，进行仪器检查和冲洗。涂片治疗组如下：无菌蒸馏水；5.25％次氯酸钠; Saver®; 17％EDTA。根样品是盲的。通过扫描电子显微镜收集根样品的显微图像。使用显微照片上的图像分析软件对开放的牙本质小管的百分比进行定量。使用双向方差分析和Tukey的多重比较（p <.05）。EDTA的17％显示牙本质小管开放百分比最高，比其他治疗方法（p <.01）。在所有根部区域，平均17％EDTA的开放性牙本质小管百分比显示出与Saver®处理相比有统计学上的显着差异（p <.01）。在蒸馏水冲洗中，牙本质小管被致密的涂抹层覆盖，而在次氯酸盐冲洗中未观察到牙本质结构的管状模式。商业化的Saver®产品在涂抹层上的清洁性能比17％EDTA差，并且与经过测试的同类产品相比，涂抹层的去除没有任何改善。
更新日期：2020-09-09
中文翻译：
扫描电镜评估5％乙二胺四乙酸去除涂片层的功效。
该研究的目的是评估新颖的Saver®冲洗溶液（包括5％EDTA）的去污层去除功效，并比较17％EDTA作为最常用的冲洗溶液。将四十四根单根牙齿分为四组，进行仪器检查和冲洗。涂片治疗组如下：无菌蒸馏水；5.25％次氯酸钠; Saver®; 17％EDTA。根样品是盲的。通过扫描电子显微镜收集根样品的显微图像。使用显微照片上的图像分析软件对开放的牙本质小管的百分比进行定量。使用双向方差分析和Tukey的多重比较（p <.05）。EDTA的17％显示牙本质小管开放百分比最高，比其他治疗方法（p <.01）。在所有根部区域，平均17％EDTA的开放性牙本质小管百分比显示出与Saver®处理相比有统计学上的显着差异（p <.01）。在蒸馏水冲洗中，牙本质小管被致密的涂抹层覆盖，而在次氯酸盐冲洗中未观察到牙本质结构的管状模式。商业化的Saver®产品在涂抹层上的清洁性能比17％EDTA差，并且与经过测试的同类产品相比，涂抹层的去除没有任何改善。
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