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Zinc‐protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-09-09 , DOI: 10.1002/bmc.4983
Nathália R Santos 1 , Matheus de J Bandeira 1 , Homègnon A Ferreol Bah 1 , Juliana L G Rodrigues 1 , Mariana S Cardoso 1 , Andrea R Rocha 2 , José A Menezes-Filho 1, 2
Affiliation  

Lead (Pb) exposure compromises heme synthesis by inhibiting ferrochelatase, forming zinc‐protoporphyrin (ZnPP). This study aims to validate a method for the determination of ZnPP by HPLC with fluorescence detection and apply this method to evaluate the extent of Pb exposure of artisanal pottery workers. Extraction procedures were tested using both nonacid and acid liquid–liquid extraction. The former presented a better chromatogram and recovery results. The validated method yielded a good resolution of ZnPP and its free form peaks with acceptable precision and accuracy. Total run time was 15 min and ZnPP peak retention time was 5.6 min. We applied this method to evaluate 39 potters (90% male), mean age 40 years (9–80). The medians (ranges) of blood lead, ZnPP and hemoglobin were 16.0 μg/dl (2.2–71.5), 12.6 μg/dl (4.6–279.8) and 15.1 g/dl (11.0–17.8), respectively. Significant differences were observed for blood lead according to gender, age range (>40 years), direct handling of lead oxide and years of occupation. Significantly higher levels of ZnPP were observed in male potters involved with lead glazing activity. The validated method was shown to be simple with one‐step nonacid extraction, good sensitivity, reproducibility and accuracy. Our data shows that these laborers are dangerously exposed to Pb, reflecting the effect on the heme synthesis.

中文翻译:

高效液相色谱法测定锌原卟啉,荧光检测是手工陶器工人铅效应的生物标志物

铅(Pb)暴露会通过抑制铁螯合酶,形成锌原卟啉(ZnPP)损害血红素的合成。这项研究的目的是验证一种通过HPLC和荧光检测法测定ZnPP的方法,并将其用于评估手工陶工的Pb暴露程度。使用非酸和酸液-液萃取对萃取程序进行了测试。前者具有更好的色谱图和回收率结果。经过验证的方法可得到良好的ZnPP分离度及其游离形式的峰,并具有可接受的精度和准确度。总运行时间为15分钟,ZnPP峰保留时间为5.6分钟。我们采用这种方法评估了39位陶工(男性占90%),平均年龄40岁(9-80岁)。血铅,ZnPP和血红蛋白的中位数(范围)为16.0μg/ dl(2.2–71.5),12.6μg/ dl(4.6–279.8)和15.1 g / dl(11.0–17)。8)。根据性别,年龄范围(> 40岁),直接接触氧化铅和职业年限,血铅存在显着差异。在与铅上釉活性有关的雄性陶工中观察到了更高水平的ZnPP。经验证的方法简单,一步法非酸提取,灵敏度高,重现性和准确性高。我们的数据表明,这些劳动者危险地暴露于Pb,反映出对血红素合成的影响。经验证的方法简单,一步法非酸提取,灵敏度高,重现性和准确性高。我们的数据表明,这些劳动者危险地暴露于Pb,反映出对血红素合成的影响。经验证的方法简单,一步法非酸提取,灵敏度高,重现性和准确性高。我们的数据表明,这些劳动者危险地暴露于Pb,反映出对血红素合成的影响。
更新日期:2020-09-09
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