Intrinsic Apyrase-Like Activity of Cerium-Based Metal-Organic Frameworks: Dephosphorylation of Adenosine Tri- and Diphosphate.,Angewandte Chemie International Edition

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Intrinsic Apyrase-Like Activity of Cerium-Based Metal-Organic Frameworks: Dephosphorylation of Adenosine Tri- and Diphosphate.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-09-09 , DOI: 10.1002/anie.202008259
Jian Yang ₁ , Ke Li ₁ , Chunzhong Li ₁ , Jinlou Gu ₁

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Apyrase is an important family of extracellular enzymes that catalyse the hydrolysis of high‐energy phosphate bonds (HEPBs) in ATP and ADP, thereby modulating many physiological processes and driving life activities. Herein, we report an unexpected discovery that cerium‐based metal–organic frameworks (Ce‐MOFs) of UiO‐66(Ce) have intrinsic apyrase‐like activity for ATP/ADP‐related physiological processes. The abundant Ce^III/Ce^IV couple sites of Ce‐MOFs endow them with the ability to selectively catalyse the hydrolysis of HEPBs of ATP and ADP under physiological conditions. Compared to natural enzymes, they could resist extreme pH and temperature, and present a broad range of working conditions. Based on this finding, a significant inhibitory effect on ADP‐induced platelet aggregation was observed upon exposing the platelet‐rich plasma (PRP) to the biomimetic UiO‐66(Ce) films, prefiguring their wide application potentials in medicine and biotechnology.

中文翻译：

铈基金属有机骨架的内在磷酸酶活性：三磷酸腺苷和二磷酸腺苷的去磷酸化。

Apyrase是重要的细胞外酶家族，可催化ATP和ADP中高能磷酸键（HEPB）的水解，从而调节许多生理过程并推动生命活动。在此，我们报告了一个意想不到的发现，即UiO-66（Ce）的铈基金属有机骨架（Ce-MOFs）具有与ATP / ADP相关的生理过程的内切性腺苷二磷酸酶活性。丰富的Ce ^III / Ce ^IVCe-MOF的两个位点赋予它们在生理条件下选择性催化ATP和ADP的HEPB水解的能力。与天然酶相比，它们可以抵抗极端的pH和温度，并具有广泛的工作条件。基于这一发现，将富含血小板的血浆（PRP）暴露于仿生UiO-66（Ce）膜后，观察到了对ADP诱导的血小板聚集的显着抑制作用，预示了它们在医学和生物技术中的广泛应用潜力。

更新日期：2020-09-09

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