Abstract Various chemical agents are used in the treatment of Non-Small Cell Lung Cancer (NSCLC). 2′-methylklavuzon was proposed as a potential chemotherapeutic agent in cancer treatment based on its topoisomerase inhibition activity. In this study the cellular effects of 2′-methylklavuzon was evaluated on A549 cancer cells using FTIR spectroscopy. 2′-methylklavuzon induced significant changes on both the whole cell lyophilizates and the lipid extracts of the A549 lung cancer cells. 2′-methylklavuzon caused significant structural changes in A549 cell DNA structure: T, A and G DNA breathing modes are lost after the drug application indicating the loss of topoisomerase activity. The level of transcription and RNA synthesis was enhanced. 2′-methylklavuzon induced single stranded DNA formation evidenced by the increase in the ratio of asymmetric/symmetric phosphate stretching modes. 2′-methylklavuzon induced band shifts only in the asymmetric mode of phosphate bonds not in the symmetrical phosphate bond stretching. 2′-methylklavuzon induced A form of DNA topography. In addition to the changes in the DNA structure and transcription 2′-methylklavuzon also caused lipid-lowering effect in A549 cancer cells. 2′-methylklavuzon suppressed lipid unsaturation, however, it induced formation of lipids with ring structures. 2′-methylklavuzon suppressed phosphate-containing lipids significantly and decreased carbonyl containing lipids and cholesterol slightly. 2′-methylklavuzon caused increases in the hydrocarbon chain length. Overall, 2′-methylklavuzon can be used as a lipid-lowering compound in the treatment of NSCLC and other cancer therapies.

中文翻译：

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2'-甲基克拉维松对 A549 非小细胞肺癌细胞具有降脂作用，并通过傅里叶变换红外光谱证实 DNA 结构发生显着变化

摘要 多种化学药剂用于治疗非小细胞肺癌 (NSCLC)。基于其拓扑异构酶抑制活性，2'-甲基克拉维松被提议作为癌症治疗中的潜在化学治疗剂。在这项研究中，使用 FTIR 光谱评估了 2'-甲基克拉维松对 A549 癌细胞的细胞作用。2'-甲基克拉维松对 A549 肺癌细胞的全细胞冻干物和脂质提取物均诱导显着变化。2'-甲基克拉维宗引起 A549 细胞 DNA 结构的显着结构变化：T、A 和 G DNA 呼吸模式在药物应用后消失，表明拓扑异构酶活性丧失。转录和RNA合成水平得到提高。2'-甲基克拉维松诱导单链 DNA 形成，这可以通过不对称/对称磷酸酯拉伸模式比率的增加来证明。2'-甲基克拉维松仅在磷酸键的不对称模式中引起带位移，而不是在对称的磷酸键拉伸中。2'-methylklavuzon 诱导了一种形式的 DNA 拓扑。除了 DNA 结构和转录的变化外，2'-甲基克拉维松还在 A549 癌细胞中引起了降脂作用。2'-甲基克拉维宗抑制脂质不饱和，然而，它诱导具有环状结构的脂质的形成。2'-甲基克拉维松显着抑制含磷酸盐的脂质，并轻微降低含羰基的脂质和胆固醇。2'-甲基克拉维宗导致烃链长度增加。全面的，