Soil denitrification produces the potent greenhouse gas nitrous oxide (N₂O) and by further reduction of N₂O, the harmless inert gas N₂. N₂O emission is determined by rate and timing of the N₂O producing and reducing steps which are sensitive to a series of proximal and distal regulators such as pH and microbial community composition. Microbial community associations to N₂O emission potential (N₂O/(N₂O + N₂)) are commonly entangled with pH leaving the true role of community composition unclear. Here, we leverage a set of soil microbiomes strongly linked to rainfall above pH to test the hypothesis that microbiome vs. N₂O emission potential (N₂O/(N₂O + N₂)) correlations will be maintained across alternative distal drivers. N₂O emission potential (N₂O/(N₂O + N₂)) and denitrification gas (NO, N₂O, N₂) kinetics were assessed by automated gas chromatography while community composition was assessed by 16 S rRNA gene sequencing and qPCR of nosZI and II genes. Analyses revealed a sustained correlation between microbiome and N₂O emission potential (N₂O/(N₂O + N₂)) in the absence of a pH effect. Further, a continuum of gas accumulation phenotypes linked to NO accumulation and sensitive to carbon addition are identified. Separate phenotypes carried out N₂O production and reduction steps more concurrently or sequentially and thus determined N₂O accumulation and emission potential (N₂O/(N₂O + N₂)). Concurrent N₂O producing/reducing soils typically contained NO accumulation to a low steady state, while carbon addition manipulations which increased NO accumulation also increased sequentiality of N₂O production/reduction and thus emission potential (N₂O/(N₂O + N₂)). These features may indicate a conserved NO inhibitory mechanism across multiple effectors (rainfall, community composition, carbon availability).

土壤反硝化产生强效的温室气体一氧化二氮（N ₂ O），并通过进一步还原N ₂ O（无害惰性气体N _2）。N ₂ O的排放量取决于对一系列近端和远端调节剂（例如pH值和微生物群落组成）敏感的N ₂ O产生和还原步骤的速率和时间。微生物群落与N ₂ O排放潜能的关系（N ₂ O /（N ₂ O + N ₂））通常与pH纠缠在一起，但不清楚社区组成的真正作用。在这里，我们利用一组土壤微生物群与pH值以上的降雨密切相关，以检验以下假设：在替代的远端驱动因素中微生物群与N ₂ O的排放潜力（N ₂ O /（N ₂ O + N ₂））之间的相关性将得以维持。 。通过自动气相色谱法评估N ₂ O排放势（N ₂ O /（N ₂ O + N ₂））和反硝化气体（NO，N ₂ O，N ₂）动力学，同时通过16 S rRNA基因测序评估群落组成和定量PCR nosZI和II基因。分析表明，在没有pH效应的情况下，微生物组与N ₂ O发射电位（N ₂ O /（N ₂ O + N ₂））之间存在持续的相关性。此外，鉴定了与NO积累相关并且对碳添加敏感的气体积累表型的连续体。分开的表型更同时或依次进行N ₂ O的产生和还原步骤，从而确定N ₂ O的积累和发射电位（N ₂ O /（N ₂ O + N ₂））。并发N ₂产生或还原O的土壤通常将NO的积累保持在较低的稳态，而增加NO积累的碳添加操作也会增加N ₂ O的产生/还原的顺序，从而增加排放电位（N ₂ O /（N ₂ O + N ₂） ）。这些特征可能表明跨多个效应子的保守的NO抑制机制（降雨，群落组成，碳有效性）。