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A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea.
Journal of Microbiological Methods ( IF 2.2 ) Pub Date : 2020-09-09 , DOI: 10.1016/j.mimet.2020.106053
Dong Xuan Nguyen 1 , Takehito Nakazawa 2 , Genki Myo 2 , Chikako Inoue 2 , Moriyuki Kawauchi 2 , Masahiro Sakamoto 2 , Yoichi Honda 2
Affiliation  

A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation experiments.



中文翻译:

一种启动子检测系统,该系统使用针对平菇和灰霉菌的基因靶向。

使用基因靶向方法,在有或没有CRISPR / Cas9技术的情况下,开发了一种新的针对姬松茸的启动子检测方法。它可以精确评估染色体原始位点的启动子活性,而无需在常规转化实验中进行随机和多次整合。

更新日期:2020-11-09
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