Algal biofuels are a promising alternative to fossil fuels, but their widespread use is hindered by problems with mass production. Light-emitting diodes (LEDs) with specific light wavelengths could be used as an energy source for algal growth and lipid synthesis. In this study, the effects of light source on the biomass and lipid production of the aerial microalga Coccomyxa sp. KGU-D001 were evaluated using LEDs. The integration of two-phase cultures, including growth and lipid production under the stress of nitrate depletion, was assessed for efficient lipid production under liquid- or aerial-phase conditions. Different wavelengths of light (blue, green, and red) were tested under liquid- and aerial-phase conditions. Under aerial-phase culture, the fatty acid contents in biofilm reached 320 mg g DWC⁻¹ with the red LEDs. In view of these findings, we describe a one-step culture method for growth and lipid accumulation in algal biofilm under aerial-phase culture with red LED irradiation. When Coccomyxa biofilm was cultured on wet cotton wool with BBM in a petri dish under the red LED, it was able to grow and accumulate lipids under the aerial-phase condition. Based on the results of this study, a potential method for a continuous biodiesel production system is proposed.

藻类生物燃料是一种很有前景的化石燃料替代品，但其广泛使用受到大规模生产问题的阻碍。具有特定光波长的发光二极管 (LED) 可用作藻类生长和脂质合成的能源。在这项研究中，光源对气生微藻Coccomyxa sp.的生物量和脂质生产的影响。KGU-D001 使用 LED 进行评估。两阶段培养的整合，包括硝酸盐消耗胁迫下的生长和脂质生产，评估了液相或空气相条件下的有效脂质生产。在液相和空气相条件下测试了不同波长的光（蓝色、绿色和红色）。气生培养下，生物膜脂肪酸含量达到320 mg·g DWC^-1带有红色 LED。鉴于这些发现，我们描述了一种一步培养方法，用于在红色 LED 照射下的气生相培养下藻类生物膜中的生长和脂质积累。当Coccomyxa生物膜在带有 BBM 的湿棉毛上在红色 LED 的培养皿中培养时，它能够在气相条件下生长和积累脂质。基于这项研究的结果，提出了一种用于连续生物柴油生产系统的潜在方法。