The nucleotide binding oligomerization domain like receptors, or NOD like receptors (NLRs), are intracellular receptors responsible for recognizing pathogens in vertebrates. Several NLR mammalian models have been characterized and analyzed but few studies have been performed with teleost species. In this study, we analyzed the nucleotide sequence of six mRNA variants of NLRC3 in Atlantic salmon (SsNLRC3), and we deduced the amino acid sequence coding for two different isoforms with a total length of 1135 amino acids and 1093 amino acids. We analyzed the phylogeny of all variants, including a Piscirickettsia salmonis infection in Atlantic salmon. All variants and their expression pattern during infection were analyzed using real-time qPCR. One of the analyzed variants was over-expressed during the early stages of Piscirickettsia salmonis infection, and we were able to identify two different SsNLRC3 isoforms. Lastly, we observed that an alteration in the amino acid sequence of one of the isoforms can directly affect the pathogen recognition function.

核苷酸结合寡聚结构域样受体或 NOD 样受体 (NLR) 是负责识别脊椎动物病原体的细胞内受体。已经对几种 NLR 哺乳动物模型进行了表征和分析，但很少对硬骨鱼物种进行研究。在这项研究中，我们分析了大西洋鲑（SsNLRC3）中NLRC3的6个mRNA变异体的核苷酸序列，并推断出编码两种不同亚型的氨基酸序列，总长度为1135个氨基酸和1093个氨基酸。我们分析了所有变种的系统发育，包括鲑鱼鱼大西洋鲑的感染。使用实时 qPCR 分析感染期间的所有变体及其表达模式。一种分析的变体在鲑鱼感染的早期阶段过度表达，我们能够识别出两种不同的 SsNLRC3 同种型。最后，我们观察到其中一种亚型的氨基酸序列的改变可以直接影响病原体识别功能。