Abstract All-female populations of mandarin fish (Siniperca chuatsi) provide economic advantages in production because of the superior growth of females relative to males. The first step to produce all-female populations was artificially induced sex reversal of female mandarin fish; beginning at 10 dph, fish (undifferentiated) were treated with dietary 17α-methyltestosterone (MT) at 50 mg/kg and 100 mg/kg dosages for 2 months. The MT treatments successfully produced all-male stocks (male rate 100%), as compared with the control group (51.11%). The survival rate of MT-treated and control groups was not significantly different. MT did not alter fish growth over the course of the experiment to 240 dph. MT treatment promoted the development of testes, and it initially inhibited gonadosomatic index (GSI) and the levels of serum steroid hormone (T and E2). Nevertheless, at the 180 dph and 240 dph, the GSI and serum steroid hormone (T and E2) levels in MT treatment groups had no significant difference with control groups. The masculinization of mandarin fish was also demonstrated by the expression patterns of sex-specific genes, dmrt1, sox9, foxl2 and cyp19a1a; the gonads of MT-treated fish exclusively expressed male-specific dmrt1 and sox9 with no expression of female-specific foxl2 and cyp19a1a.

中文翻译：

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口服17α-甲基睾酮雄性化生产新雄性鳜鱼

摘要 全雌性鳜鱼 (Siniperca chuatsi) 种群在生产方面具有经济优势，因为雌性相对于雄性的生长速度更快。产生全雌性种群的第一步是人工诱导雌性鳜鱼的性别逆转；从 10 dph 开始，用 50 mg/kg 和 100 mg/kg 剂量的膳食 17α-甲基睾酮 (MT) 处理鱼（未分化）2 个月。与对照组 (51.11%) 相比，MT 处理成功地产生了全雄性种群 (雄性比率 100%)。MT 治疗组和对照组的存活率没有显着差异。在 240 dph 的实验过程中，MT 没有改变鱼的生长。MT治疗促进睾丸发育，它最初抑制性腺体指数 (GSI) 和血清类固醇激素 (T 和 E2) 的水平。尽管如此，在 180 dph 和 240 dph 时，MT 治疗组的 GSI 和血清类固醇激素（T 和 E2）水平与对照组没有显着差异。鳜鱼的男性化也通过性别特异性基因 dmrt1、sox9、foxl2 和 cyp19a1a 的表达模式得到证实；MT 处理的鱼的性腺只表达雄性特异性 dmrt1 和 sox9，不表达雌性特异性 foxl2 和 cyp19a1a。foxl2 和 cyp19a1a；MT 处理的鱼的性腺只表达雄性特异性 dmrt1 和 sox9，不表达雌性特异性 foxl2 和 cyp19a1a。foxl2 和 cyp19a1a；MT 处理的鱼的性腺只表达雄性特异性 dmrt1 和 sox9，不表达雌性特异性 foxl2 和 cyp19a1a。