A signal "off-on" electrochemiluminescence resonance energy transfer (ECL-RET) sensor based on carboxylated graphene-like carbon nitride (C-g-C3N4) as donor and CuO nanoneedles as acceptor has been constructed. The distance between donor and acceptor is a critical factor for ECL-RET sensors. Herein, we used a new method to make CuO nanoneedles grow in situ on C-g-C3N4 to form a nanocomposite, largely reducing the distance between donor and acceptor and greatly improving ECL-RET efficiency. In this system, because the CuO could be reduced by dopamine (DA), the ECL emission was significantly enhanced. Hence, a sensitive ECL sensor was successfully fabricated for quantitative detection of DA in dopamine hydrochloride injection and human serum sample. Further, the ECL-RET sensor exhibited a wide linear range from 10 nM to 1 mM, as well as a low detection limit of 8.2 nM. With its excellent stability and selectivity, the novel strategy will enable numerous applications in biological systems.

中文翻译：

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基于 Cg-C3N4/CuO 纳米复合材料中高效共振能量转移的新型“关断”电化学发光传感器

已经构建了一种基于羧化石墨烯类氮化碳 (Cg-C3N4) 作为供体和 CuO 纳米针作为受体的信号“关断”电化学发光共振能量转移 (ECL-RET) 传感器。供体和受体之间的距离是 ECL-RET 传感器的关键因素。在此，我们使用一种新方法使 CuO 纳米针在 Cg-C3N4 上原位生长形成纳米复合材料，大大缩短了供体和受体之间的距离，大大提高了 ECL-RET 效率。在该系统中，由于多巴胺 (DA) 可以减少 CuO，因此 ECL 发射显着增强。因此，成功地制造了一种灵敏的 ECL 传感器，用于定量检测盐酸多巴胺注射液和人血清样品中的 DA。此外，ECL-RET 传感器表现出从 10 nM 到 1 mM 的宽线性范围，以及 8.2 nM 的低检测限。凭借其出色的稳定性和选择性，这种新颖的策略将使生物系统中的众多应用成为可能。