Ensuring the clonal identity of accessions within a germplasm repository, such as the US National Pyrus collection, is critical. Additionally, pedigree confirmation and understanding population structure is an important part of breeding and managing genetic resources. The ability to validate pedigree and identity is challenging and inconclusive through morphology alone. DNA information can be used to confirm parentage and identity by descent. A Pyrus fingerprinting set was previously developed by the European Cooperative Program for Plant Genetic Resources (ECPGR) and consists of 12 dinucleotide-containing simple sequence repeats (SSRs) markers that are amplified in two PCR reactions. The ECPGR set is difficult to use because dinucleotide-containing SSRs often exhibit a number of amplification artifacts such as stutters, split peaks, and binning errors. High-core repeat (3–6 bp motifs) SSRs do not exhibit many of the artifacts displayed by dinucleotide-containing SSRs. Therefore, an easy-to-use 10-SSR fingerprint set containing high-core repeat SSRs that can be evaluated in a single reaction was developed. This fingerprinting set, known as the US Pyrus Genetic Resources (USPGR) set, was compared with the ECPGR set and to single nucleotide polymorphism (SNP) markers found on the new 70K pear Axiom™ array for its ability to assess diversity, population structure, pedigree, and identity. The USPGR set performed similarly to both genotyping platforms while being easier to use. Additionally, the present study demonstrates the usefulness of SSRs in the age of high-throughput genotyping and sequencing platforms.

确保种质库（例如美国国家Pyrus馆藏）内种质的克隆身份至关重要。此外，家谱确认和了解种群结构是遗传资源育种和管理的重要组成部分。仅通过形态学，验证血统和身份的能力就具有挑战性和不确定性。DNA信息可用于确认血统和身份。一个梨指纹图谱集先前是由欧洲植物遗传资源合作计划（ECPGR）开发的，由12个含二核苷酸的简单序列重复（SSR）标记组成，这些标记在两个PCR反应中得到了扩增。ECPGR集很难使用，因为含二核苷酸的SSR经常表现出许多扩增伪影，例如口吃，分裂峰和合并错误。高核重复序列（3–6 bp的基序）SSR并不表现出许多包含二核苷酸的SSR所显示的伪像。因此，开发了一种易于使用的10-SSR指纹集，其中包含可以在单个反应中评估的高核重复SSR。此指纹集，称为美国Pyrus将遗传资源（USPGR）集与ECPGR集以及新的70K梨Axiom™阵列上发现的单核苷酸多态性（SNP）标记进行了比较，以评估其多样性，种群结构，谱系和同一性。USPGR装置的性能类似于两个基因分型平台，但更易于使用。此外，本研究证明了在高通量基因分型和测序平台时代，SSR的有用性。