The main objective of this study was to evaluate the effects of sodium sulfite (SoS) treatment of maize and its impact on the porcine immune system in the presence of an LPS-induced systemic inflammation. Control maize (CON) and Fusarium-toxin contaminated maize (FUS) were wet-preserved (20% moisture) for 79 days with (+) or without (−) SoS and then included at 10% in a diet, resulting in four experimental groups: CON−, CON+, FUS−, and FUS+ with deoxynivalenol (DON) concentrations of 0.09, 0.05, 5.36, and 0.83 mg DON/kg feed, respectively. After 42-day feeding trial (weaned barrows, n = 20/group), ten pigs per group were challenged intraperitoneally with either 7.5 μg LPS/kg BW or placebo (0.9% NaCl), observed for 2 h, and then sacrificed. Blood, mesenteric lymph nodes, and spleen were collected for phenotyping of different T cell subsets, B cells, and monocytes. Phagocytic activity and intracellular formation of reactive oxygen species (ROS) were analyzed in both polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC) using flow cytometry. Our results revealed that the impact of DON was more notable on CD3⁺CD4⁺CD8⁺ T cells in lymphoid tissues rather than in blood T cells. In contrast, SoS treatment of maize altered leukocyte subpopulations in blood, e.g., reduced the percentage and fluorescence signal of CD8^high T cells. Interestingly, SoS treatment reduced the amount of free radicals in basal ROS-producing PMNs only in LPS-challenged animals, suggesting a decrease in basal cellular ROS production (p_SoS*LPS = 0.022).

这项研究的主要目的是评估在LPS诱导的系统性炎症存在下，亚硫酸钠（SoS）处理玉米的效果及其对猪免疫系统的影响。对照玉米（CON）和受镰刀菌毒素污染的玉米（FUS）在有（+）或没有（-）SoS的情况下湿保存（20％水分）79天，然后以10％的饮食添加，进行了四个实验组：CON-，CON +，FUS-和FUS +，其中脱氧雪腐烯醇（DON）的浓度分别为0.09、0.05、5.36和0.83 mg DON / kg饲料。经过42天的喂养试验（断奶的公猪，n= 20 /组），每组十只猪腹膜内接受7.5μgLPS / kg BW或安慰剂（0.9％NaCl）攻击，观察2小时，然后处死。收集血液，肠系膜淋巴结和脾脏，用于不同T细胞亚群，B细胞和单核细胞的表型分析。使用流式细胞仪分析了多形核细胞（PMN）和外周血单核细胞（PBMC）的吞噬活性和活性氧物种（ROS）的细胞内形成。我们的结果表明，DON对淋巴组织而不是血液T细胞中CD3 ⁺ CD4 ⁺ CD8 ⁺ T细胞的影响更为明显。相反，SoS处理玉米会改变血液中的白细胞亚群，例如降低CD8的百分比和荧光信号^高T细胞。有趣的是，SoS处理仅在LPS攻击的动物中减少了产生基础ROS的PMN中自由基的量，这表明基础细胞ROS的产生减少了（p _{SoS * LPS} = 0.022）。