Abstract Vulvovaginal candidiasis is one of the frequent vaginal infections in women caused by Candida albicans . We evaluate the expression of ERG11 , CDR1 and CDR2 genes by quantitative RT-PCR in Candida albicans strains isolated from women in north of Iran. Of 50 samples, 23 (46%) were determined to be Candida albicans strains. In phenotypic assays, 20 (~87%) of strains were resistant to doses of fluconazole (≥128–2048 μg/mL). Also, 43.47 and 26.08% of isolates were resistant to ketoconazole and clotrimazole, respectively. Overexpression or mutation of ERG11 and upregulation of CDR1 and CDR2 genes was found in some resistant isolates compared to strain ATCC10231. Multidrug resistance in in the isolates was due to the upregulation of abovementioned genes as well as decreased affinity of ERG11 to azoles. Thus, we need to new strategies and drugs to treat vulvovaginitis in Guilan’s women.

中文翻译：

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外阴阴道念珠菌病女性白色念珠菌分离株耐药性的分子方面

摘要 外阴阴道念珠菌病是由白色念珠菌引起的女性常见阴道感染之一。我们通过定量 RT-PCR 在从伊朗北部妇女分离的白色念珠菌菌株中评估了 ERG11、CDR1 和 CDR2 基因的表达。在 50 个样品中，23 个 (46%) 被确定为白色念珠菌菌株。在表型分析中，20 (~87%) 菌株对氟康唑剂量 (≥128–2048 μg/mL) 具有抗性。此外，分别有 43.47% 和 26.08% 的分离株对酮康唑和克霉唑耐药。与菌株 ATCC10231 相比，在一些耐药菌株中发现了 ERG11 的过度表达或突变以及 CDR1 和 CDR2 基因的上调。分离株中的多药耐药性是由于上述基因的上调以及 ERG11 对唑类的亲和力降低。因此，