Trehalose-6-phosphate phosphatase (TPP) plays a key role in trehalose metabolism in plants. Here, we performed comprehensive in silico analyses and identified 12 OsTPPs (Oryza sativa TPPs) utilizing various bioinformatics tools. Phylogenetic tree, accomplished with OsTPPs and TPPs from 11 monocot and dicot species, was divided mainly into two clades, each clade containing six OsTPPs. Exon–intron distribution was related to phylogenetic clades. All OsTPPs are distributed within nine chromosomes (chr.), except Chr. 1, Chr. 5 and Chr. 11. OsTPPs were found to be stable in nature according to the 3-D structure prediction. Cis-regulatory elements (CREs) were also analyzed using 2 kb upstream of start codon for each gene to predict their biological functions. We categorized all CREs in five distinct groups based on core elements, stress response, cellular development, hormonal regulation, and unknown function, distributed in a range of 3–14 CREs in each group. Interestingly, our expression analysis showed that OsTPPs were more upregulated in response to drought and cold stresses compared to salt stress. Abundance of stress-related CREs found signifies TPPs’ possible role in stress response, which may facilitate to find related transcription factors and unveil complex molecular mechanisms during stress response.

6-海藻糖磷酸酶（TPP）在植物海藻糖代谢中起关键作用。在这里，我们进行了在硅片分析全面，确定了12个OsTPPs（水稻的TPP）利用各种生物信息学工具。用来自11个单子叶植物和双子叶植物物种的OsTPP和TPP完成的系统发育树主要分为两个进化枝，每个进化枝包含6个OsTPP。外显子-内含子分布与进化进化枝有关。除Chr外，所有OsTPP均分布在9个染色体内。1，Chr。5和Chr。11.根据3-D结构预测，发现OsTPP本质上是稳定的。顺式还使用起始密码子上游2 kb的每个基因分析了调控元件（CRE），以预测其生物学功能。根据核心要素，应激反应，细胞发育，激素调节和未知功能，我们将所有CRE分为五个不同的组，每组分布在3-14个CRE中。有趣的是，我们的表达分析表明，与盐胁迫相比，OsTPPs在干旱和寒冷胁迫下的反应更加上调。发现大量与压力有关的CREs表示TPPs在压力反应中的可能作用，这可能有助于寻找相关的转录因子并揭示压力反应过程中的复杂分子机制。